Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily

Lily (<i>Lilium</i> spp.) is a widely cultivated horticultural crop that has high ornamental and commercial value but also the serious problem of pollen pollution. However, mechanisms of anther dehiscence in lily remain largely unknown. In this study, the morphological characteristics of...

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Autores principales:	Ling He, Xinyue Liu, Ze Wu, Nianjun Teng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lilium</i> spp. anther dehiscence stomium degeneration transcriptome metabolome Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1d644f7ca4b149bcb9a97dd54e655be8
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spelling	oai:doaj.org-article:1d644f7ca4b149bcb9a97dd54e655be82021-11-25T17:53:35ZTranscriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily10.3390/ijms2222121241422-00671661-6596https://doaj.org/article/1d644f7ca4b149bcb9a97dd54e655be82021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12124https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Lily (<i>Lilium</i> spp.) is a widely cultivated horticultural crop that has high ornamental and commercial value but also the serious problem of pollen pollution. However, mechanisms of anther dehiscence in lily remain largely unknown. In this study, the morphological characteristics of the stomium zone (SZ) from different developmental stages of ‘Siberia’ lily anthers were investigated. In addition, transcriptomic and metabolomic data were analyzed to identify the differentially expressed genes (DEGs) and secondary metabolites involved in stomium degeneration. According to morphological observations, SZ lysis occurred when flower buds were 6–8 cm in length and was completed in 9 cm. Transcriptomic analysis identified the genes involved in SZ degeneration, including those associated with hormone signal transduction, cell structure, reactive oxygen species (ROS), and transcription factors. A weighted co-expression network showed strong correlations between transcription factors. In addition, TUNEL (TdT-mediated dUTP nick-end labeling) assays showed that programmed cell death was important during anther SZ degeneration. Jasmonates might also have key roles in anther dehiscence by affecting the expression of the genes involved in pectin lysis, water transport, and cysteine protease. Collectively, the results of this study improve our understanding of anther dehiscence in lily and provide a data platform from which the molecular mechanisms of SZ degeneration can be revealed.Ling HeXinyue LiuZe WuNianjun TengMDPI AGarticle<i>Lilium</i> spp.anther dehiscencestomium degenerationtranscriptomemetabolomeBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12124, p 12124 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lilium</i> spp. anther dehiscence stomium degeneration transcriptome metabolome Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Lilium</i> spp. anther dehiscence stomium degeneration transcriptome metabolome Biology (General) QH301-705.5 Chemistry QD1-999 Ling He Xinyue Liu Ze Wu Nianjun Teng Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
description	Lily (<i>Lilium</i> spp.) is a widely cultivated horticultural crop that has high ornamental and commercial value but also the serious problem of pollen pollution. However, mechanisms of anther dehiscence in lily remain largely unknown. In this study, the morphological characteristics of the stomium zone (SZ) from different developmental stages of ‘Siberia’ lily anthers were investigated. In addition, transcriptomic and metabolomic data were analyzed to identify the differentially expressed genes (DEGs) and secondary metabolites involved in stomium degeneration. According to morphological observations, SZ lysis occurred when flower buds were 6–8 cm in length and was completed in 9 cm. Transcriptomic analysis identified the genes involved in SZ degeneration, including those associated with hormone signal transduction, cell structure, reactive oxygen species (ROS), and transcription factors. A weighted co-expression network showed strong correlations between transcription factors. In addition, TUNEL (TdT-mediated dUTP nick-end labeling) assays showed that programmed cell death was important during anther SZ degeneration. Jasmonates might also have key roles in anther dehiscence by affecting the expression of the genes involved in pectin lysis, water transport, and cysteine protease. Collectively, the results of this study improve our understanding of anther dehiscence in lily and provide a data platform from which the molecular mechanisms of SZ degeneration can be revealed.
format	article
author	Ling He Xinyue Liu Ze Wu Nianjun Teng
author_facet	Ling He Xinyue Liu Ze Wu Nianjun Teng
author_sort	Ling He
title	Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
title_short	Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
title_full	Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
title_fullStr	Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
title_full_unstemmed	Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily
title_sort	transcriptome and metabolome analyses provide insights into the stomium degeneration mechanism in lily
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1d644f7ca4b149bcb9a97dd54e655be8
work_keys_str_mv	AT linghe transcriptomeandmetabolomeanalysesprovideinsightsintothestomiumdegenerationmechanisminlily AT xinyueliu transcriptomeandmetabolomeanalysesprovideinsightsintothestomiumdegenerationmechanisminlily AT zewu transcriptomeandmetabolomeanalysesprovideinsightsintothestomiumdegenerationmechanisminlily AT nianjunteng transcriptomeandmetabolomeanalysesprovideinsightsintothestomiumdegenerationmechanisminlily
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Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily

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