A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.

Hemagglutinin (HA) is the immunodominant protein of the influenza virus. We previously showed that mice injected with a monoglycosylated influenza A HA (HAmg) produced cross-strain-reactive antibodies and were better protected than mice injected with a fully glycosylated HA (HAfg) during lethal dose...

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Autores principales:	Yi-An Ko, Yueh-Hsiang Yu, Yen-Fei Wu, Yung-Chieh Tseng, Chia-Lin Chen, King-Siang Goh, Hsin-Yu Liao, Ting-Hua Chen, Ting-Jen Rachel Cheng, An-Suei Yang, Chi-Huey Wong, Che Ma, Kuo-I Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1d68c84373464a6bb70bde385675c643
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spelling	oai:doaj.org-article:1d68c84373464a6bb70bde385675c6432021-12-02T20:00:25ZA non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.1553-73661553-737410.1371/journal.ppat.1009724https://doaj.org/article/1d68c84373464a6bb70bde385675c6432021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009724https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Hemagglutinin (HA) is the immunodominant protein of the influenza virus. We previously showed that mice injected with a monoglycosylated influenza A HA (HAmg) produced cross-strain-reactive antibodies and were better protected than mice injected with a fully glycosylated HA (HAfg) during lethal dose challenge. We employed a single B-cell screening platform to isolate the cross-protective monoclonal antibody (mAb) 651 from mice immunized with the HAmg of A/Brisbane/59/2007 (H1N1) influenza virus (Bris/07). The mAb 651 recognized the head domain of a broad spectrum of HAs from groups 1 and 2 influenza A viruses and offered prophylactic and therapeutic efficacy against A/California/07/2009 (H1N1) (Cal/09) and Bris/07 infections in mice. The antibody did not possess neutralizing activity; however, antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis mediated by natural killer cells and alveolar macrophages were important in the protective efficacy of mAb 651. Together, this study highlighted the significance of effector functions for non-neutralizing antibodies to exhibit protection against influenza virus infection.Yi-An KoYueh-Hsiang YuYen-Fei WuYung-Chieh TsengChia-Lin ChenKing-Siang GohHsin-Yu LiaoTing-Hua ChenTing-Jen Rachel ChengAn-Suei YangChi-Huey WongChe MaKuo-I LinPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009724 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Yi-An Ko Yueh-Hsiang Yu Yen-Fei Wu Yung-Chieh Tseng Chia-Lin Chen King-Siang Goh Hsin-Yu Liao Ting-Hua Chen Ting-Jen Rachel Cheng An-Suei Yang Chi-Huey Wong Che Ma Kuo-I Lin A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
description	Hemagglutinin (HA) is the immunodominant protein of the influenza virus. We previously showed that mice injected with a monoglycosylated influenza A HA (HAmg) produced cross-strain-reactive antibodies and were better protected than mice injected with a fully glycosylated HA (HAfg) during lethal dose challenge. We employed a single B-cell screening platform to isolate the cross-protective monoclonal antibody (mAb) 651 from mice immunized with the HAmg of A/Brisbane/59/2007 (H1N1) influenza virus (Bris/07). The mAb 651 recognized the head domain of a broad spectrum of HAs from groups 1 and 2 influenza A viruses and offered prophylactic and therapeutic efficacy against A/California/07/2009 (H1N1) (Cal/09) and Bris/07 infections in mice. The antibody did not possess neutralizing activity; however, antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis mediated by natural killer cells and alveolar macrophages were important in the protective efficacy of mAb 651. Together, this study highlighted the significance of effector functions for non-neutralizing antibodies to exhibit protection against influenza virus infection.
format	article
author	Yi-An Ko Yueh-Hsiang Yu Yen-Fei Wu Yung-Chieh Tseng Chia-Lin Chen King-Siang Goh Hsin-Yu Liao Ting-Hua Chen Ting-Jen Rachel Cheng An-Suei Yang Chi-Huey Wong Che Ma Kuo-I Lin
author_facet	Yi-An Ko Yueh-Hsiang Yu Yen-Fei Wu Yung-Chieh Tseng Chia-Lin Chen King-Siang Goh Hsin-Yu Liao Ting-Hua Chen Ting-Jen Rachel Cheng An-Suei Yang Chi-Huey Wong Che Ma Kuo-I Lin
author_sort	Yi-An Ko
title	A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
title_short	A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
title_full	A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
title_fullStr	A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
title_full_unstemmed	A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
title_sort	non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1d68c84373464a6bb70bde385675c643
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A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells.

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