Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses

Abstract Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical anal...

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Autores principales:	GeonHui Lee, JuKyung Lee, JeongHoon Kim, Hak Soo Choi, Jonghan Kim, SangHoon Lee, HeaYeon Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d6cddb501f04d8b94bd9b0e91d1a76e
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spelling	oai:doaj.org-article:1d6cddb501f04d8b94bd9b0e91d1a76e2021-12-02T15:05:54ZSingle Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses10.1038/s41598-017-06144-92045-2322https://doaj.org/article/1d6cddb501f04d8b94bd9b0e91d1a76e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06144-9https://doaj.org/toc/2045-2322Abstract Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.GeonHui LeeJuKyung LeeJeongHoon KimHak Soo ChoiJonghan KimSangHoon LeeHeaYeon LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q GeonHui Lee JuKyung Lee JeongHoon Kim Hak Soo Choi Jonghan Kim SangHoon Lee HeaYeon Lee Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
description	Abstract Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.
format	article
author	GeonHui Lee JuKyung Lee JeongHoon Kim Hak Soo Choi Jonghan Kim SangHoon Lee HeaYeon Lee
author_facet	GeonHui Lee JuKyung Lee JeongHoon Kim Hak Soo Choi Jonghan Kim SangHoon Lee HeaYeon Lee
author_sort	GeonHui Lee
title	Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
title_short	Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
title_full	Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
title_fullStr	Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
title_full_unstemmed	Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses
title_sort	single microfluidic electrochemical sensor system for simultaneous multi-pulmonary hypertension biomarker analyses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1d6cddb501f04d8b94bd9b0e91d1a76e
work_keys_str_mv	AT geonhuilee singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT jukyunglee singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT jeonghoonkim singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT haksoochoi singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT jonghankim singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT sanghoonlee singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses AT heayeonlee singlemicrofluidicelectrochemicalsensorsystemforsimultaneousmultipulmonaryhypertensionbiomarkeranalyses
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Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses

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