Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis
Abstract The possibility of detecting and classifying living cells in a label-free and non-invasive manner holds significant theranostic potential. In this work, Hyperspectral Imaging (HSI) has been successfully applied to the analysis of macrophagic polarization, given its central role in several p...
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2017
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oai:doaj.org-article:1d75aee3c71e4d898809e9c4699d76772021-12-02T11:41:00ZClassification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis10.1038/s41598-017-08121-82045-2322https://doaj.org/article/1d75aee3c71e4d898809e9c4699d76772017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08121-8https://doaj.org/toc/2045-2322Abstract The possibility of detecting and classifying living cells in a label-free and non-invasive manner holds significant theranostic potential. In this work, Hyperspectral Imaging (HSI) has been successfully applied to the analysis of macrophagic polarization, given its central role in several pathological settings, including the regulation of tumour microenvironment. Human monocyte derived macrophages have been investigated using hyperspectral reflectance confocal microscopy, and hyperspectral datasets have been analysed in terms of M1 vs. M2 polarization by Principal Components Analysis (PCA). Following PCA, Linear Discriminant Analysis has been implemented for semi-automatic classification of macrophagic polarization from HSI data. Our results confirm the possibility to perform single-cell-level in vitro classification of M1 vs. M2 macrophages in a non-invasive and label-free manner with a high accuracy (above 98% for cells deriving from the same donor), supporting the idea of applying the technique to the study of complex interacting cellular systems, such in the case of tumour-immunity in vitro models.Francesca R. BertaniPamela MozeticMarco FioramontiMichele IulianiGiulia RibelliFrancesco PantanoDaniele SantiniGiuseppe ToniniMarcella TrombettaLuca BusinaroStefano SelciAlberto RainerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
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Medicine R Science Q Francesca R. Bertani Pamela Mozetic Marco Fioramonti Michele Iuliani Giulia Ribelli Francesco Pantano Daniele Santini Giuseppe Tonini Marcella Trombetta Luca Businaro Stefano Selci Alberto Rainer Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| description |
Abstract The possibility of detecting and classifying living cells in a label-free and non-invasive manner holds significant theranostic potential. In this work, Hyperspectral Imaging (HSI) has been successfully applied to the analysis of macrophagic polarization, given its central role in several pathological settings, including the regulation of tumour microenvironment. Human monocyte derived macrophages have been investigated using hyperspectral reflectance confocal microscopy, and hyperspectral datasets have been analysed in terms of M1 vs. M2 polarization by Principal Components Analysis (PCA). Following PCA, Linear Discriminant Analysis has been implemented for semi-automatic classification of macrophagic polarization from HSI data. Our results confirm the possibility to perform single-cell-level in vitro classification of M1 vs. M2 macrophages in a non-invasive and label-free manner with a high accuracy (above 98% for cells deriving from the same donor), supporting the idea of applying the technique to the study of complex interacting cellular systems, such in the case of tumour-immunity in vitro models. |
| format |
article |
| author |
Francesca R. Bertani Pamela Mozetic Marco Fioramonti Michele Iuliani Giulia Ribelli Francesco Pantano Daniele Santini Giuseppe Tonini Marcella Trombetta Luca Businaro Stefano Selci Alberto Rainer |
| author_facet |
Francesca R. Bertani Pamela Mozetic Marco Fioramonti Michele Iuliani Giulia Ribelli Francesco Pantano Daniele Santini Giuseppe Tonini Marcella Trombetta Luca Businaro Stefano Selci Alberto Rainer |
| author_sort |
Francesca R. Bertani |
| title |
Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| title_short |
Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| title_full |
Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| title_fullStr |
Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| title_full_unstemmed |
Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| title_sort |
classification of m1/m2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/1d75aee3c71e4d898809e9c4699d7677 |
| work_keys_str_mv |
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