Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate

Summary: Intracellular signaling dynamics play fundamental roles in cell biology. Precise modulation of the amplitude, duration, and frequency of signaling activation will be a powerful approach to investigate molecular mechanisms as well as to engineer signaling to control cell behaviors. Here, we...

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Autores principales:	Liang Guo, Kan Zhu, Michael Pargett, Adam Contreras, Patrick Tsai, Quan Qing, Wolfgang Losert, John Albeck, Min Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biotechnology Cell biology Integrative aspects of cell biology Functional aspects of cell biology Science Q
Acceso en línea:	https://doaj.org/article/1d7951c8fef84b53b75c23fe71a219f8
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spelling	oai:doaj.org-article:1d7951c8fef84b53b75c23fe71a219f82021-11-20T05:08:46ZElectrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate2589-004210.1016/j.isci.2021.103240https://doaj.org/article/1d7951c8fef84b53b75c23fe71a219f82021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012086https://doaj.org/toc/2589-0042Summary: Intracellular signaling dynamics play fundamental roles in cell biology. Precise modulation of the amplitude, duration, and frequency of signaling activation will be a powerful approach to investigate molecular mechanisms as well as to engineer signaling to control cell behaviors. Here, we showed a practical approach to achieve precise amplitude modulation (AM), frequency modulation (FM), and duration modulation (DM) of MAP kinase activation. Alternating current (AC) electrical stimulation induced synchronized ERK activation. Amplitude and duration of ERK activation were controlled by varying stimulation strength and duration. ERK activation frequencies were arbitrarily modulated with trains of short AC applications with accurately defined intervals. Significantly, ERK dynamics coded by well-designed AC can rewire PC12 cell fate independent of growth factors. This technique can be used to synchronize and modulate ERK activation dynamics, thus would offer a practical way to control cell behaviors in vivo without the use of biochemical agents or genetic manipulation.Liang GuoKan ZhuMichael PargettAdam ContrerasPatrick TsaiQuan QingWolfgang LosertJohn AlbeckMin ZhaoElsevierarticleBiotechnologyCell biologyIntegrative aspects of cell biologyFunctional aspects of cell biologyScienceQENiScience, Vol 24, Iss 11, Pp 103240- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology Cell biology Integrative aspects of cell biology Functional aspects of cell biology Science Q
spellingShingle	Biotechnology Cell biology Integrative aspects of cell biology Functional aspects of cell biology Science Q Liang Guo Kan Zhu Michael Pargett Adam Contreras Patrick Tsai Quan Qing Wolfgang Losert John Albeck Min Zhao Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
description	Summary: Intracellular signaling dynamics play fundamental roles in cell biology. Precise modulation of the amplitude, duration, and frequency of signaling activation will be a powerful approach to investigate molecular mechanisms as well as to engineer signaling to control cell behaviors. Here, we showed a practical approach to achieve precise amplitude modulation (AM), frequency modulation (FM), and duration modulation (DM) of MAP kinase activation. Alternating current (AC) electrical stimulation induced synchronized ERK activation. Amplitude and duration of ERK activation were controlled by varying stimulation strength and duration. ERK activation frequencies were arbitrarily modulated with trains of short AC applications with accurately defined intervals. Significantly, ERK dynamics coded by well-designed AC can rewire PC12 cell fate independent of growth factors. This technique can be used to synchronize and modulate ERK activation dynamics, thus would offer a practical way to control cell behaviors in vivo without the use of biochemical agents or genetic manipulation.
format	article
author	Liang Guo Kan Zhu Michael Pargett Adam Contreras Patrick Tsai Quan Qing Wolfgang Losert John Albeck Min Zhao
author_facet	Liang Guo Kan Zhu Michael Pargett Adam Contreras Patrick Tsai Quan Qing Wolfgang Losert John Albeck Min Zhao
author_sort	Liang Guo
title	Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
title_short	Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
title_full	Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
title_fullStr	Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
title_full_unstemmed	Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate
title_sort	electrically synchronizing and modulating the dynamics of erk activation to regulate cell fate
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1d7951c8fef84b53b75c23fe71a219f8
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_version_	1718419528446115840

Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate

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