Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

Jin-Bao Qin,1,5,* Kang-An Li,2,* Xiang-Xiang Li,1,5 Qing-Song Xie,3 Jia-Ying Lin,4 Kai-Chuang Ye,1,5 Mi-Er Jiang,1,5 Gui-Xiang Zhang,2 Xin-Wu Lu1,51Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 2Departmen...

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Autores principales:	Qin JB, Li KA, Li XX, Xie QS, Lin JY, Ye KC, Jiang ME, Zhang GX, Lu XW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1d7ec7adb9ca439186ec8eedea092d69
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spelling	oai:doaj.org-article:1d7ec7adb9ca439186ec8eedea092d692021-12-02T07:37:04ZLong-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury1176-91141178-2013https://doaj.org/article/1d7ec7adb9ca439186ec8eedea092d692012-10-01T00:00:00Zhttp://www.dovepress.com/long-term-mri-tracking-of-dual-labeled-adipose-derived-stem-cells-homi-a11162https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jin-Bao Qin,1,5,* Kang-An Li,2,* Xiang-Xiang Li,1,5 Qing-Song Xie,3 Jia-Ying Lin,4 Kai-Chuang Ye,1,5 Mi-Er Jiang,1,5 Gui-Xiang Zhang,2 Xin-Wu Lu1,51Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 2Department of Radiology, Shanghai First People&#39;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 3Department of Neurosurgery, Cixi Municipal People&#39;s Hospital, Zhejiang Province, China; 4Clinic for Gynecology, Charite-Universitatsmedizin Berlin, Berlin, Germany; 5Vascular Center, Shanghai Jiao Tong University, Shanghai, China*These two authors contributed equally to this workBackground: Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury.Methods: GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation.Results: GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging.Conclusion: Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.Keywords: adipose-derived stem cells, carotid artery injury, magnetic resonance imaging, iron oxide particles, cell therapyQin JBLi KALi XXXie QSLin JYYe KCJiang MEZhang GXLu XWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5191-5203 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Qin JB Li KA Li XX Xie QS Lin JY Ye KC Jiang ME Zhang GX Lu XW Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
description	Jin-Bao Qin,1,5,* Kang-An Li,2,* Xiang-Xiang Li,1,5 Qing-Song Xie,3 Jia-Ying Lin,4 Kai-Chuang Ye,1,5 Mi-Er Jiang,1,5 Gui-Xiang Zhang,2 Xin-Wu Lu1,51Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 2Department of Radiology, Shanghai First People&#39;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 3Department of Neurosurgery, Cixi Municipal People&#39;s Hospital, Zhejiang Province, China; 4Clinic for Gynecology, Charite-Universitatsmedizin Berlin, Berlin, Germany; 5Vascular Center, Shanghai Jiao Tong University, Shanghai, China*These two authors contributed equally to this workBackground: Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury.Methods: GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation.Results: GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging.Conclusion: Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.Keywords: adipose-derived stem cells, carotid artery injury, magnetic resonance imaging, iron oxide particles, cell therapy
format	article
author	Qin JB Li KA Li XX Xie QS Lin JY Ye KC Jiang ME Zhang GX Lu XW
author_facet	Qin JB Li KA Li XX Xie QS Lin JY Ye KC Jiang ME Zhang GX Lu XW
author_sort	Qin JB
title	Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
title_short	Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
title_full	Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
title_fullStr	Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
title_full_unstemmed	Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
title_sort	long-term mri tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/1d7ec7adb9ca439186ec8eedea092d69
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Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

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