Microfluidics: A Novel Approach for Dehydration Protein Droplets

The equation of state of colloids plays an important role in the modelling and comprehension of industrial processes, defining the working conditions of processes such as drying, filtration, and mixing. The determination of the equation is based on the solvent equilibration, by dialysis, between the...

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Autores principales:	Van Nhat Pham, Dimitri Radajewski, Isaac Rodríguez-Ruiz, Sebastien Teychene
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microfluidics lysozyme dehydration equation of state Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1d90d2024b234b628b4a59145f2c3b49
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spelling	oai:doaj.org-article:1d90d2024b234b628b4a59145f2c3b492021-11-25T16:55:40ZMicrofluidics: A Novel Approach for Dehydration Protein Droplets10.3390/bios111104602079-6374https://doaj.org/article/1d90d2024b234b628b4a59145f2c3b492021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/460https://doaj.org/toc/2079-6374The equation of state of colloids plays an important role in the modelling and comprehension of industrial processes, defining the working conditions of processes such as drying, filtration, and mixing. The determination of the equation is based on the solvent equilibration, by dialysis, between the colloidal suspension and a reservoir with a known osmotic pressure. In this paper, we propose a novel microfluidic approach to determine the equation of state of a lysozyme solution. Monodispersed droplets of lysozyme were generated in the bulk of a continuous 1-decanol phase using a flow-focusing microfluidic geometry. In this multiphasic system and in the working operation conditions, the droplets can be considered to act as a permeable membrane system. A water mass transfer flow occurs by molecule continuous diffusion in the surrounding 1-decanol phase until a thermodynamic equilibrium is reached in a few seconds to minutes, in contrast with the standard osmotic pressure measurements. By changing the water saturation of the continuous phase, the equation of state of lysozyme in solution was determined through the relation of the osmotic pressure between protein molecules and the volume fraction of protein inside the droplets. The obtained equation shows good agreement with other standard approaches reported in the literature.Van Nhat PhamDimitri RadajewskiIsaac Rodríguez-RuizSebastien TeycheneMDPI AGarticlemicrofluidicslysozymedehydrationequation of stateBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 460, p 460 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfluidics lysozyme dehydration equation of state Biotechnology TP248.13-248.65
spellingShingle	microfluidics lysozyme dehydration equation of state Biotechnology TP248.13-248.65 Van Nhat Pham Dimitri Radajewski Isaac Rodríguez-Ruiz Sebastien Teychene Microfluidics: A Novel Approach for Dehydration Protein Droplets
description	The equation of state of colloids plays an important role in the modelling and comprehension of industrial processes, defining the working conditions of processes such as drying, filtration, and mixing. The determination of the equation is based on the solvent equilibration, by dialysis, between the colloidal suspension and a reservoir with a known osmotic pressure. In this paper, we propose a novel microfluidic approach to determine the equation of state of a lysozyme solution. Monodispersed droplets of lysozyme were generated in the bulk of a continuous 1-decanol phase using a flow-focusing microfluidic geometry. In this multiphasic system and in the working operation conditions, the droplets can be considered to act as a permeable membrane system. A water mass transfer flow occurs by molecule continuous diffusion in the surrounding 1-decanol phase until a thermodynamic equilibrium is reached in a few seconds to minutes, in contrast with the standard osmotic pressure measurements. By changing the water saturation of the continuous phase, the equation of state of lysozyme in solution was determined through the relation of the osmotic pressure between protein molecules and the volume fraction of protein inside the droplets. The obtained equation shows good agreement with other standard approaches reported in the literature.
format	article
author	Van Nhat Pham Dimitri Radajewski Isaac Rodríguez-Ruiz Sebastien Teychene
author_facet	Van Nhat Pham Dimitri Radajewski Isaac Rodríguez-Ruiz Sebastien Teychene
author_sort	Van Nhat Pham
title	Microfluidics: A Novel Approach for Dehydration Protein Droplets
title_short	Microfluidics: A Novel Approach for Dehydration Protein Droplets
title_full	Microfluidics: A Novel Approach for Dehydration Protein Droplets
title_fullStr	Microfluidics: A Novel Approach for Dehydration Protein Droplets
title_full_unstemmed	Microfluidics: A Novel Approach for Dehydration Protein Droplets
title_sort	microfluidics: a novel approach for dehydration protein droplets
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1d90d2024b234b628b4a59145f2c3b49
work_keys_str_mv	AT vannhatpham microfluidicsanovelapproachfordehydrationproteindroplets AT dimitriradajewski microfluidicsanovelapproachfordehydrationproteindroplets AT isaacrodriguezruiz microfluidicsanovelapproachfordehydrationproteindroplets AT sebastienteychene microfluidicsanovelapproachfordehydrationproteindroplets
_version_	1718412823433838592

Microfluidics: A Novel Approach for Dehydration Protein Droplets

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