Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states.
Amoeboid locomotion is one of the typical modes of biological cell migration. Cytoplasmic sol-gel conversion of an actomyosin system is thought to play an important role in locomotion. However, the mechanisms underlying sol-gel conversion, including trigger, signal, and regulating factors, remain un...
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:1d917e0f63db4531bf9c98832a191a812021-11-18T09:01:23ZReconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states.1932-620310.1371/journal.pone.0070317https://doaj.org/article/1d917e0f63db4531bf9c98832a191a812013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940560/?tool=EBIhttps://doaj.org/toc/1932-6203Amoeboid locomotion is one of the typical modes of biological cell migration. Cytoplasmic sol-gel conversion of an actomyosin system is thought to play an important role in locomotion. However, the mechanisms underlying sol-gel conversion, including trigger, signal, and regulating factors, remain unclear. We developed a novel model system in which an actomyosin fraction moves like an amoeba in a cytoplasmic extract. Rheological study of this model system revealed that the actomyosin fraction exhibits shear banding: the sol-gel state of actomyosin can be regulated by shear rate or mechanical force. Furthermore, study of the living cell indicated that the shear-banding property also causes sol-gel conversion with the same order of magnitude as that of shear rate. Our results suggest that the inherent sol-gel transition property plays an essential role in the self-regulation of autonomous translational motion in amoeba.Yukinori NishigamiMasatoshi IchikawaToshiya KazamaRyo KobayashiTeruo ShimmenKenichi YoshikawaSeiji SonobePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70317 (2013) |
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DOAJ |
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DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Yukinori Nishigami Masatoshi Ichikawa Toshiya Kazama Ryo Kobayashi Teruo Shimmen Kenichi Yoshikawa Seiji Sonobe Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| description |
Amoeboid locomotion is one of the typical modes of biological cell migration. Cytoplasmic sol-gel conversion of an actomyosin system is thought to play an important role in locomotion. However, the mechanisms underlying sol-gel conversion, including trigger, signal, and regulating factors, remain unclear. We developed a novel model system in which an actomyosin fraction moves like an amoeba in a cytoplasmic extract. Rheological study of this model system revealed that the actomyosin fraction exhibits shear banding: the sol-gel state of actomyosin can be regulated by shear rate or mechanical force. Furthermore, study of the living cell indicated that the shear-banding property also causes sol-gel conversion with the same order of magnitude as that of shear rate. Our results suggest that the inherent sol-gel transition property plays an essential role in the self-regulation of autonomous translational motion in amoeba. |
| format |
article |
| author |
Yukinori Nishigami Masatoshi Ichikawa Toshiya Kazama Ryo Kobayashi Teruo Shimmen Kenichi Yoshikawa Seiji Sonobe |
| author_facet |
Yukinori Nishigami Masatoshi Ichikawa Toshiya Kazama Ryo Kobayashi Teruo Shimmen Kenichi Yoshikawa Seiji Sonobe |
| author_sort |
Yukinori Nishigami |
| title |
Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| title_short |
Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| title_full |
Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| title_fullStr |
Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| title_full_unstemmed |
Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| title_sort |
reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/1d917e0f63db4531bf9c98832a191a81 |
| work_keys_str_mv |
AT yukinorinishigami reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT masatoshiichikawa reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT toshiyakazama reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT ryokobayashi reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT teruoshimmen reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT kenichiyoshikawa reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates AT seijisonobe reconstructionofactiveregularmotioninamoebaextractdynamiccooperationbetweensolandgelstates |
| _version_ |
1718421010188861440 |