Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

Abstract Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largel...

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Autores principales:	Jingyi Liu, Nan Mei, Yaling Wang, Xinjin Shi, Hongjun Chen
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Fowl adenovirus Hexon protein Monoclonal antibody Linear epitope Biotechnology TP248.13-248.65 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1d9c38ee54ee480a870de501472982ec
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spelling	oai:doaj.org-article:1d9c38ee54ee480a870de501472982ec2021-11-21T12:09:06ZIdentification of a novel immunological epitope on Hexon of fowl adenovirus serotype 410.1186/s13568-021-01309-22191-0855https://doaj.org/article/1d9c38ee54ee480a870de501472982ec2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13568-021-01309-2https://doaj.org/toc/2191-0855Abstract Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.Jingyi LiuNan MeiYaling WangXinjin ShiHongjun ChenSpringerOpenarticleFowl adenovirusHexon proteinMonoclonal antibodyLinear epitopeBiotechnologyTP248.13-248.65MicrobiologyQR1-502ENAMB Express, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fowl adenovirus Hexon protein Monoclonal antibody Linear epitope Biotechnology TP248.13-248.65 Microbiology QR1-502
spellingShingle	Fowl adenovirus Hexon protein Monoclonal antibody Linear epitope Biotechnology TP248.13-248.65 Microbiology QR1-502 Jingyi Liu Nan Mei Yaling Wang Xinjin Shi Hongjun Chen Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
description	Abstract Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.
format	article
author	Jingyi Liu Nan Mei Yaling Wang Xinjin Shi Hongjun Chen
author_facet	Jingyi Liu Nan Mei Yaling Wang Xinjin Shi Hongjun Chen
author_sort	Jingyi Liu
title	Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
title_short	Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
title_full	Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
title_fullStr	Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
title_full_unstemmed	Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
title_sort	identification of a novel immunological epitope on hexon of fowl adenovirus serotype 4
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/1d9c38ee54ee480a870de501472982ec
work_keys_str_mv	AT jingyiliu identificationofanovelimmunologicalepitopeonhexonoffowladenovirusserotype4 AT nanmei identificationofanovelimmunologicalepitopeonhexonoffowladenovirusserotype4 AT yalingwang identificationofanovelimmunologicalepitopeonhexonoffowladenovirusserotype4 AT xinjinshi identificationofanovelimmunologicalepitopeonhexonoffowladenovirusserotype4 AT hongjunchen identificationofanovelimmunologicalepitopeonhexonoffowladenovirusserotype4
_version_	1718419171640868864

Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

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