An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.

Cardiac tissue macrophages (cTMs) are a previously uncharacterised cell type that we have identified and characterise here as an abundant GFP(+) population within the adult Cx(3)cr1(GFP/+) knock-in mouse heart. They comprise the predominant myeloid cell population in the myocardium, and are found th...

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Autores principales:	Alexander R Pinto, Rosa Paolicelli, Ekaterina Salimova, Janko Gospocic, Esfir Slonimsky, Daniel Bilbao-Cortes, James W Godwin, Nadia A Rosenthal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d9e9e5177264f1ab53748c38590a33a
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spelling	oai:doaj.org-article:1d9e9e5177264f1ab53748c38590a33a2021-11-18T07:19:10ZAn abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.1932-620310.1371/journal.pone.0036814https://doaj.org/article/1d9e9e5177264f1ab53748c38590a33a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22590615/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cardiac tissue macrophages (cTMs) are a previously uncharacterised cell type that we have identified and characterise here as an abundant GFP(+) population within the adult Cx(3)cr1(GFP/+) knock-in mouse heart. They comprise the predominant myeloid cell population in the myocardium, and are found throughout myocardial interstitial spaces interacting directly with capillary endothelial cells and cardiomyocytes. Flow cytometry-based immunophenotyping shows that cTMs exhibit canonical macrophage markers. Gene expression analysis shows that cTMs (CD45(+)CD11b(+)GFP(+)) are distinct from mononuclear CD45(+)CD11b(+)GFP(+) cells sorted from the spleen and brain of adult Cx(3)cr1(GFP/+) mice. Gene expression profiling reveals that cTMs closely resemble alternatively-activated anti-inflammatory M2 macrophages, expressing a number of M2 markers, including Mrc1, CD163, and Lyve-1. While cTMs perform normal tissue macrophage homeostatic functions, they also exhibit a distinct phenotype, involving secretion of salutary factors (including IGF-1) and immune modulation. In summary, the characterisation of cTMs at the cellular and molecular level defines a potentially important role for these cells in cardiac homeostasis.Alexander R PintoRosa PaolicelliEkaterina SalimovaJanko GospocicEsfir SlonimskyDaniel Bilbao-CortesJames W GodwinNadia A RosenthalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36814 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander R Pinto Rosa Paolicelli Ekaterina Salimova Janko Gospocic Esfir Slonimsky Daniel Bilbao-Cortes James W Godwin Nadia A Rosenthal An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
description	Cardiac tissue macrophages (cTMs) are a previously uncharacterised cell type that we have identified and characterise here as an abundant GFP(+) population within the adult Cx(3)cr1(GFP/+) knock-in mouse heart. They comprise the predominant myeloid cell population in the myocardium, and are found throughout myocardial interstitial spaces interacting directly with capillary endothelial cells and cardiomyocytes. Flow cytometry-based immunophenotyping shows that cTMs exhibit canonical macrophage markers. Gene expression analysis shows that cTMs (CD45(+)CD11b(+)GFP(+)) are distinct from mononuclear CD45(+)CD11b(+)GFP(+) cells sorted from the spleen and brain of adult Cx(3)cr1(GFP/+) mice. Gene expression profiling reveals that cTMs closely resemble alternatively-activated anti-inflammatory M2 macrophages, expressing a number of M2 markers, including Mrc1, CD163, and Lyve-1. While cTMs perform normal tissue macrophage homeostatic functions, they also exhibit a distinct phenotype, involving secretion of salutary factors (including IGF-1) and immune modulation. In summary, the characterisation of cTMs at the cellular and molecular level defines a potentially important role for these cells in cardiac homeostasis.
format	article
author	Alexander R Pinto Rosa Paolicelli Ekaterina Salimova Janko Gospocic Esfir Slonimsky Daniel Bilbao-Cortes James W Godwin Nadia A Rosenthal
author_facet	Alexander R Pinto Rosa Paolicelli Ekaterina Salimova Janko Gospocic Esfir Slonimsky Daniel Bilbao-Cortes James W Godwin Nadia A Rosenthal
author_sort	Alexander R Pinto
title	An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
title_short	An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
title_full	An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
title_fullStr	An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
title_full_unstemmed	An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
title_sort	abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1d9e9e5177264f1ab53748c38590a33a
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An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.

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