Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal

Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction. Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO). Taguchi method was applied in the optimiz...

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Autores principales:	Lian Zhong, Jin Li, Dawei Tian, Jun Cai, Han Wang, Qimin Ma
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	chitosan immobilization phenolic compounds polyphenol oxidase rectorite Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/1da0527451984a12b1c538c098901b46
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spelling	oai:doaj.org-article:1da0527451984a12b1c538c098901b462021-11-06T10:48:10ZImmobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal0273-12231996-973210.2166/wst.2021.024https://doaj.org/article/1da0527451984a12b1c538c098901b462021-02-01T00:00:00Zhttp://wst.iwaponline.com/content/83/4/906https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction. Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO). Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively. APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO. The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored. The results indicated that APPO was more efficient in phenolic compounds removal than CPPO. APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively. The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively. The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.Lian ZhongJin LiDawei TianJun CaiHan WangQimin MaIWA Publishingarticlechitosanimmobilizationphenolic compoundspolyphenol oxidaserectoriteEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 4, Pp 906-921 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitosan immobilization phenolic compounds polyphenol oxidase rectorite Environmental technology. Sanitary engineering TD1-1066
spellingShingle	chitosan immobilization phenolic compounds polyphenol oxidase rectorite Environmental technology. Sanitary engineering TD1-1066 Lian Zhong Jin Li Dawei Tian Jun Cai Han Wang Qimin Ma Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
description	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction. Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO). Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively. APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO. The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored. The results indicated that APPO was more efficient in phenolic compounds removal than CPPO. APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively. The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively. The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
format	article
author	Lian Zhong Jin Li Dawei Tian Jun Cai Han Wang Qimin Ma
author_facet	Lian Zhong Jin Li Dawei Tian Jun Cai Han Wang Qimin Ma
author_sort	Lian Zhong
title	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
title_short	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
title_full	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
title_fullStr	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
title_full_unstemmed	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
title_sort	immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/1da0527451984a12b1c538c098901b46
work_keys_str_mv	AT lianzhong immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval AT jinli immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval AT daweitian immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval AT juncai immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval AT hanwang immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval AT qiminma immobilizationofpolyphenoloxidaseonchitosanorganicrectoritecompositesforphenoliccompoundsremoval
_version_	1718443779922329600

Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal

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