Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography

Objective: Purification of immunoglobulin Y from egg yolk by chromatography is a new investigation. In this study, we developed an easy and inexpensive method for purification of IgY from egg yolk. Methods: The yolk from egg was separated and passed through a nylon mesh into a measuring cylinder. Th...

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Autores principales:	M Pour Amir, MJ Rasaei
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2001
Materias:	immunoglobulin y (igy) gel filtration purification Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1da1526633c04117b56960651eddacad
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spelling	oai:doaj.org-article:1da1526633c04117b56960651eddacad2021-11-10T09:21:00ZPurification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography1561-41072251-7170https://doaj.org/article/1da1526633c04117b56960651eddacad2001-04-01T00:00:00Zhttp://jbums.org/article-1-2868-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: Purification of immunoglobulin Y from egg yolk by chromatography is a new investigation. In this study, we developed an easy and inexpensive method for purification of IgY from egg yolk. Methods: The yolk from egg was separated and passed through a nylon mesh into a measuring cylinder. The egg yolk was diluted 10 times with HCL (3mM) and prepared suspension was centrifuged. Solid ammonium sulfate was added to 60% saturation. The precipitate was dissolved in phosphate buffer and applied on the gel-filtration columns (Sephadex G25, G200). Findings: The result of cellulose acetate electrophoresis at pH=9 showed some extra bands which were isolated with ammonium sulfate precipitation and sephadex G-25 chromatography. The purified band was observed in the Y–globulin region. After sephadex G-200 chromatography, SDS-PAGE electrophoresis of the product showed on band about 63KD. Conclusion: IgY extraction and purification with acid, centrifugation, ammonium sulfate and gel filtration are effective and simple operative methods.M Pour AmirMJ RasaeiBabol University of Medical Sciencesarticleimmunoglobulin y (igy)gel filtrationpurificationMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 3, Iss 2, Pp 7-11 (2001)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	immunoglobulin y (igy) gel filtration purification Medicine R Medicine (General) R5-920
spellingShingle	immunoglobulin y (igy) gel filtration purification Medicine R Medicine (General) R5-920 M Pour Amir MJ Rasaei Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
description	Objective: Purification of immunoglobulin Y from egg yolk by chromatography is a new investigation. In this study, we developed an easy and inexpensive method for purification of IgY from egg yolk. Methods: The yolk from egg was separated and passed through a nylon mesh into a measuring cylinder. The egg yolk was diluted 10 times with HCL (3mM) and prepared suspension was centrifuged. Solid ammonium sulfate was added to 60% saturation. The precipitate was dissolved in phosphate buffer and applied on the gel-filtration columns (Sephadex G25, G200). Findings: The result of cellulose acetate electrophoresis at pH=9 showed some extra bands which were isolated with ammonium sulfate precipitation and sephadex G-25 chromatography. The purified band was observed in the Y–globulin region. After sephadex G-200 chromatography, SDS-PAGE electrophoresis of the product showed on band about 63KD. Conclusion: IgY extraction and purification with acid, centrifugation, ammonium sulfate and gel filtration are effective and simple operative methods.
format	article
author	M Pour Amir MJ Rasaei
author_facet	M Pour Amir MJ Rasaei
author_sort	M Pour Amir
title	Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
title_short	Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
title_full	Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
title_fullStr	Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
title_full_unstemmed	Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography
title_sort	purification of immunoglobulin y (igy) from egg yolk by gel filtration chromatography
publisher	Babol University of Medical Sciences
publishDate	2001
url	https://doaj.org/article/1da1526633c04117b56960651eddacad
work_keys_str_mv	AT mpouramir purificationofimmunoglobulinyigyfromeggyolkbygelfiltrationchromatography AT mjrasaei purificationofimmunoglobulinyigyfromeggyolkbygelfiltrationchromatography
_version_	1718440080381575168

Purification of immunoglobulin Y (IgY) from egg yolk by gel filtration chromatography

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