Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids

Abstract Extracellular vesicles (EVs) derived from tumor cells have the potential to provide a much-needed source of non-invasive molecular biomarkers for liquid biopsies. However, current methods for EV isolation have limited specificity towards tumor-derived EVs that limit their clinical use. Here...

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Autores principales:	Jingjing Zhang, Luong T. H. Nguyen, Richard Hickey, Nicole Walters, Xinyu Wang, Kwang Joo Kwak, L. James Lee, Andre F. Palmer, Eduardo Reátegui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1da76a5ccfd04a989da3fc36409bd677
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spelling	oai:doaj.org-article:1da76a5ccfd04a989da3fc36409bd6772021-12-02T15:51:16ZImmunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids10.1038/s41598-021-86910-y2045-2322https://doaj.org/article/1da76a5ccfd04a989da3fc36409bd6772021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86910-yhttps://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) derived from tumor cells have the potential to provide a much-needed source of non-invasive molecular biomarkers for liquid biopsies. However, current methods for EV isolation have limited specificity towards tumor-derived EVs that limit their clinical use. Here, we present an approach called immunomagnetic sequential ultrafiltration (iSUF) that consists of sequential stages of purification and enrichment of EVs in approximately 2 h. In iSUF, EVs present in different volumes of biofluids (0.5–100 mL) can be significantly enriched (up to 1000 times), with up to 99% removal of contaminating proteins (e.g., albumin). The EV recovery rate by iSUF for cell culture media (CCM), serum, and urine corresponded to 98.0% ± 3.6%, 96.0% ± 2.0% and 94.0% ± 1.9%, respectively (p > 0.05). The final step of iSUF enables the separation of tumor-specific EVs by incorporating immunomagnetic beads to target EV subpopulations. Serum from a cohort of clinical samples from metastatic breast cancer (BC) patients and healthy donors were processed by the iSUF platform and the isolated EVs from patients showed significantly higher expression levels of BC biomarkers (i.e., HER2, CD24, and miR21).Jingjing ZhangLuong T. H. NguyenRichard HickeyNicole WaltersXinyu WangKwang Joo KwakL. James LeeAndre F. PalmerEduardo ReáteguiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingjing Zhang Luong T. H. Nguyen Richard Hickey Nicole Walters Xinyu Wang Kwang Joo Kwak L. James Lee Andre F. Palmer Eduardo Reátegui Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
description	Abstract Extracellular vesicles (EVs) derived from tumor cells have the potential to provide a much-needed source of non-invasive molecular biomarkers for liquid biopsies. However, current methods for EV isolation have limited specificity towards tumor-derived EVs that limit their clinical use. Here, we present an approach called immunomagnetic sequential ultrafiltration (iSUF) that consists of sequential stages of purification and enrichment of EVs in approximately 2 h. In iSUF, EVs present in different volumes of biofluids (0.5–100 mL) can be significantly enriched (up to 1000 times), with up to 99% removal of contaminating proteins (e.g., albumin). The EV recovery rate by iSUF for cell culture media (CCM), serum, and urine corresponded to 98.0% ± 3.6%, 96.0% ± 2.0% and 94.0% ± 1.9%, respectively (p > 0.05). The final step of iSUF enables the separation of tumor-specific EVs by incorporating immunomagnetic beads to target EV subpopulations. Serum from a cohort of clinical samples from metastatic breast cancer (BC) patients and healthy donors were processed by the iSUF platform and the isolated EVs from patients showed significantly higher expression levels of BC biomarkers (i.e., HER2, CD24, and miR21).
format	article
author	Jingjing Zhang Luong T. H. Nguyen Richard Hickey Nicole Walters Xinyu Wang Kwang Joo Kwak L. James Lee Andre F. Palmer Eduardo Reátegui
author_facet	Jingjing Zhang Luong T. H. Nguyen Richard Hickey Nicole Walters Xinyu Wang Kwang Joo Kwak L. James Lee Andre F. Palmer Eduardo Reátegui
author_sort	Jingjing Zhang
title	Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
title_short	Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
title_full	Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
title_fullStr	Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
title_full_unstemmed	Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
title_sort	immunomagnetic sequential ultrafiltration (isuf) platform for enrichment and purification of extracellular vesicles from biofluids
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1da76a5ccfd04a989da3fc36409bd677
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Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids

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