Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.

Acne vulgaris is a chronic inflammatory disorder of the sebaceous follicles. Propionibacterium acnes (P. acnes), a gram-positive anareobic bacterium, plays a critical role in the development of these inflammatory lesions. This study aimed at determining whether reactive oxygen species (ROS) are prod...

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Autores principales:	Philippe A Grange, Christiane Chéreau, Joël Raingeaud, Carole Nicco, Bernard Weill, Nicolas Dupin, Frédéric Batteux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1da9b1719c3246a5bc5301fb762d436a
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spelling	oai:doaj.org-article:1da9b1719c3246a5bc5301fb762d436a2021-11-25T05:47:45ZProduction of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.1553-73661553-737410.1371/journal.ppat.1000527https://doaj.org/article/1da9b1719c3246a5bc5301fb762d436a2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19629174/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Acne vulgaris is a chronic inflammatory disorder of the sebaceous follicles. Propionibacterium acnes (P. acnes), a gram-positive anareobic bacterium, plays a critical role in the development of these inflammatory lesions. This study aimed at determining whether reactive oxygen species (ROS) are produced by keratinocytes upon P. acnes infection, dissecting the mechanism of this production, and investigating how this phenomenon integrates in the general inflammatory response induced by P. acnes. In our hands, ROS, and especially superoxide anions (O2(-)), were rapidly produced by keratinocytes upon stimulation by P. acnes surface proteins. In P. acnes-stimulated keratinocytes, O2(-) was produced by NAD(P)H oxidase through activation of the scavenger receptor CD36. O2(-) was dismuted by superoxide dismutase to form hydrogen peroxide which was further detoxified into water by the GSH/GPx system. In addition, P. acnes-induced O2(-) abrogated P. acnes growth and was involved in keratinocyte lysis through the combination of O2(-) with nitric oxide to form peroxynitrites. Finally, retinoic acid derivates, the most efficient anti-acneic drugs, prevent O2(-) production, IL-8 release and keratinocyte apoptosis, suggesting the relevance of this pathway in humans.Philippe A GrangeChristiane ChéreauJoël RaingeaudCarole NiccoBernard WeillNicolas DupinFrédéric BatteuxPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 7, p e1000527 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Philippe A Grange Christiane Chéreau Joël Raingeaud Carole Nicco Bernard Weill Nicolas Dupin Frédéric Batteux Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
description	Acne vulgaris is a chronic inflammatory disorder of the sebaceous follicles. Propionibacterium acnes (P. acnes), a gram-positive anareobic bacterium, plays a critical role in the development of these inflammatory lesions. This study aimed at determining whether reactive oxygen species (ROS) are produced by keratinocytes upon P. acnes infection, dissecting the mechanism of this production, and investigating how this phenomenon integrates in the general inflammatory response induced by P. acnes. In our hands, ROS, and especially superoxide anions (O2(-)), were rapidly produced by keratinocytes upon stimulation by P. acnes surface proteins. In P. acnes-stimulated keratinocytes, O2(-) was produced by NAD(P)H oxidase through activation of the scavenger receptor CD36. O2(-) was dismuted by superoxide dismutase to form hydrogen peroxide which was further detoxified into water by the GSH/GPx system. In addition, P. acnes-induced O2(-) abrogated P. acnes growth and was involved in keratinocyte lysis through the combination of O2(-) with nitric oxide to form peroxynitrites. Finally, retinoic acid derivates, the most efficient anti-acneic drugs, prevent O2(-) production, IL-8 release and keratinocyte apoptosis, suggesting the relevance of this pathway in humans.
format	article
author	Philippe A Grange Christiane Chéreau Joël Raingeaud Carole Nicco Bernard Weill Nicolas Dupin Frédéric Batteux
author_facet	Philippe A Grange Christiane Chéreau Joël Raingeaud Carole Nicco Bernard Weill Nicolas Dupin Frédéric Batteux
author_sort	Philippe A Grange
title	Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
title_short	Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
title_full	Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
title_fullStr	Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
title_full_unstemmed	Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.
title_sort	production of superoxide anions by keratinocytes initiates p. acnes-induced inflammation of the skin.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/1da9b1719c3246a5bc5301fb762d436a
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Production of superoxide anions by keratinocytes initiates P. acnes-induced inflammation of the skin.

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