Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging
Abstract Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been ex...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1daa93bd0b3143619402dec953db42d8 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1daa93bd0b3143619402dec953db42d8 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1daa93bd0b3143619402dec953db42d82021-12-02T15:22:57ZComprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging10.1038/s41598-021-93054-62045-2322https://doaj.org/article/1daa93bd0b3143619402dec953db42d82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93054-6https://doaj.org/toc/2045-2322Abstract Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8 µm and 0.65 µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.Ivo PlanincPatricia Garcia-CanadillaHector DejeaIvana IlicEduard GuaschMonica ZamoraFàtima CrispiMarco StampanoniDavor MilicicBart BijnensAnne BonninMaja CikesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Ivo Planinc Patricia Garcia-Canadilla Hector Dejea Ivana Ilic Eduard Guasch Monica Zamora Fàtima Crispi Marco Stampanoni Davor Milicic Bart Bijnens Anne Bonnin Maja Cikes Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| description |
Abstract Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8 µm and 0.65 µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality. |
| format |
article |
| author |
Ivo Planinc Patricia Garcia-Canadilla Hector Dejea Ivana Ilic Eduard Guasch Monica Zamora Fàtima Crispi Marco Stampanoni Davor Milicic Bart Bijnens Anne Bonnin Maja Cikes |
| author_facet |
Ivo Planinc Patricia Garcia-Canadilla Hector Dejea Ivana Ilic Eduard Guasch Monica Zamora Fàtima Crispi Marco Stampanoni Davor Milicic Bart Bijnens Anne Bonnin Maja Cikes |
| author_sort |
Ivo Planinc |
| title |
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| title_short |
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| title_full |
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| title_fullStr |
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| title_full_unstemmed |
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging |
| title_sort |
comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based x-ray phase contrast imaging |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/1daa93bd0b3143619402dec953db42d8 |
| work_keys_str_mv |
AT ivoplaninc comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT patriciagarciacanadilla comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT hectordejea comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT ivanailic comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT eduardguasch comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT monicazamora comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT fatimacrispi comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT marcostampanoni comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT davormilicic comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT bartbijnens comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT annebonnin comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging AT majacikes comprehensiveassessmentofmyocardialremodelinginischemicheartdiseasebysynchrotronpropagationbasedxrayphasecontrastimaging |
| _version_ |
1718387372594298880 |