IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS

The development of HIV vaccine remains an important goal in prophylaxis and therapy of HIV/ AIDS epidemics. There are various approaches for development of а candidate vaccine based on induction of neutralizing antibodies and cell-mediated immunity. Synthetic peptides are considered promising vaccin...

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Autores principales:	S. V. Korobova, G. V. Kornilaeva, V. A. Toporova, I. A. Nikolaeva, L. P. Trubcheninova, N. F. Trefilyeva, L. P. Sizyakina, I. G. Sidorovich, P. G. Aparin, R. M. Khaitov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	hiv peptides vaccine neutralizing antibodies mice infection enhancement Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1dad465a38384ed38d959bfb023cd5fb
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spelling	oai:doaj.org-article:1dad465a38384ed38d959bfb023cd5fb2021-11-18T08:03:45ZIMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS1563-06252313-741X10.15789/1563-0625-2016-1-51-62https://doaj.org/article/1dad465a38384ed38d959bfb023cd5fb2016-04-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/988https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe development of HIV vaccine remains an important goal in prophylaxis and therapy of HIV/ AIDS epidemics. There are various approaches for development of а candidate vaccine based on induction of neutralizing antibodies and cell-mediated immunity. Synthetic peptides are considered promising vaccine antigens since they are capable of activating both humoral and cellular immune response. HIV-1 envelope gp120 is the target for neutralizing antiviral antibodies. The V3 region of the HIV-1 gp120 is highly immunogenic and important for the virus-coreceptor interaction. In a RV144 vaccine trial, the levels of vaccine-induced IgG antibodies recognizing V1V2 regions from multiple HIV-1 subtypes show inverse correlations with a risk for HIV-1 infection. Meanwhile, HIV is characterized by high diversity. The consensus and mosaic immunogens are complete but artificial proteins, which are computationally designed to elicit immune responses with improved cross-reactive broadness. We have been studied immunogenic properties of synthetic peptides derived from V1, V2, V3 loop regions of the consensus M HIV1 (CON-S) sequence group of the gp 120 envelope protein and V3 loop derived from a Russian RUA022a2 isolate. These peptides specifically reacted to HIV-positive sera in ELISA, thus indicating their similarity to appropriate HIV proteins. The peptides proved to be weakly immunogenic. Therefore, Freund complete adjuvant was used to enhance peptide immunogenicity. To assess the immunogenicity, the mice were immunized with a peptide mixture. Antibodies have been developed to every peptide from the mixture, being, predominantly, of IgG isotype. The antibody titers depended on the length of peptide sequences. However, the sera from immunized mice did not have a HIV neutralizing activity. The serum neutralization was assessed by pseudovirus-based assay, using a molecular clone of virus isolates CAP 45.2.00.G3 and QH.209.14.M.EnvA2. The virus neutralization is a complex process and may be influenced by several factors, such as antibody titer, isotype, or antibody structure. Probably, to induce neutralizing antibodies by this peptide mixture, it is necessary to choose appropriate adjutants and immunization schedule. Moreover, it was shown that peptides could increase in vitro virus infectivity in pseudovirus-based model, using the CAP 45.2.00.G3, QH209.14M.ENV.A2, QD435.100M.ENV.E1 molecular clone. These viral isolates belong to different HIV-1 subtypes.S. V. KorobovaG. V. KornilaevaV. A. ToporovaI. A. NikolaevaL. P. TrubcheninovaN. F. TrefilyevaL. P. SizyakinaI. G. SidorovichP. G. AparinR. M. KhaitovSPb RAACIarticlehivpeptidesvaccineneutralizing antibodiesmiceinfection enhancementImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 1, Pp 51-62 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	hiv peptides vaccine neutralizing antibodies mice infection enhancement Immunologic diseases. Allergy RC581-607
spellingShingle	hiv peptides vaccine neutralizing antibodies mice infection enhancement Immunologic diseases. Allergy RC581-607 S. V. Korobova G. V. Kornilaeva V. A. Toporova I. A. Nikolaeva L. P. Trubcheninova N. F. Trefilyeva L. P. Sizyakina I. G. Sidorovich P. G. Aparin R. M. Khaitov IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
description	The development of HIV vaccine remains an important goal in prophylaxis and therapy of HIV/ AIDS epidemics. There are various approaches for development of а candidate vaccine based on induction of neutralizing antibodies and cell-mediated immunity. Synthetic peptides are considered promising vaccine antigens since they are capable of activating both humoral and cellular immune response. HIV-1 envelope gp120 is the target for neutralizing antiviral antibodies. The V3 region of the HIV-1 gp120 is highly immunogenic and important for the virus-coreceptor interaction. In a RV144 vaccine trial, the levels of vaccine-induced IgG antibodies recognizing V1V2 regions from multiple HIV-1 subtypes show inverse correlations with a risk for HIV-1 infection. Meanwhile, HIV is characterized by high diversity. The consensus and mosaic immunogens are complete but artificial proteins, which are computationally designed to elicit immune responses with improved cross-reactive broadness. We have been studied immunogenic properties of synthetic peptides derived from V1, V2, V3 loop regions of the consensus M HIV1 (CON-S) sequence group of the gp 120 envelope protein and V3 loop derived from a Russian RUA022a2 isolate. These peptides specifically reacted to HIV-positive sera in ELISA, thus indicating their similarity to appropriate HIV proteins. The peptides proved to be weakly immunogenic. Therefore, Freund complete adjuvant was used to enhance peptide immunogenicity. To assess the immunogenicity, the mice were immunized with a peptide mixture. Antibodies have been developed to every peptide from the mixture, being, predominantly, of IgG isotype. The antibody titers depended on the length of peptide sequences. However, the sera from immunized mice did not have a HIV neutralizing activity. The serum neutralization was assessed by pseudovirus-based assay, using a molecular clone of virus isolates CAP 45.2.00.G3 and QH.209.14.M.EnvA2. The virus neutralization is a complex process and may be influenced by several factors, such as antibody titer, isotype, or antibody structure. Probably, to induce neutralizing antibodies by this peptide mixture, it is necessary to choose appropriate adjutants and immunization schedule. Moreover, it was shown that peptides could increase in vitro virus infectivity in pseudovirus-based model, using the CAP 45.2.00.G3, QH209.14M.ENV.A2, QD435.100M.ENV.E1 molecular clone. These viral isolates belong to different HIV-1 subtypes.
format	article
author	S. V. Korobova G. V. Kornilaeva V. A. Toporova I. A. Nikolaeva L. P. Trubcheninova N. F. Trefilyeva L. P. Sizyakina I. G. Sidorovich P. G. Aparin R. M. Khaitov
author_facet	S. V. Korobova G. V. Kornilaeva V. A. Toporova I. A. Nikolaeva L. P. Trubcheninova N. F. Trefilyeva L. P. Sizyakina I. G. Sidorovich P. G. Aparin R. M. Khaitov
author_sort	S. V. Korobova
title	IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
title_short	IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
title_full	IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
title_fullStr	IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
title_full_unstemmed	IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS
title_sort	immunological characteristic of synthetic peptides similar to actual hiv antigen determinants
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/1dad465a38384ed38d959bfb023cd5fb
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IMMUNOLOGICAL CHARACTERISTIC OF SYNTHETIC PEPTIDES SIMILAR TO ACTUAL HIV ANTIGEN DETERMINANTS

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