A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda
Women with HIV may experience higher rates of non-AIDS comorbidities compared to men with HIV, but the underlying mechanisms are not well understood. We investigated sex-related differences in the effects of HIV on monocyte phenotypes within the Ugandan Study of HIV effects on the Myocardium and Ath...
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2021
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oai:doaj.org-article:1db156596af344d59b92edcb667ef9e72021-11-25T19:12:34ZA Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda10.3390/v131121351999-4915https://doaj.org/article/1db156596af344d59b92edcb667ef9e72021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2135https://doaj.org/toc/1999-4915Women with HIV may experience higher rates of non-AIDS comorbidities compared to men with HIV, but the underlying mechanisms are not well understood. We investigated sex-related differences in the effects of HIV on monocyte phenotypes within the Ugandan Study of HIV effects on the Myocardium and Atherosclerosis (mUTIMA). Of 133 participants who provided blood for flow cytometry assays, 86 (65%) were women and 91 (68%) were persons living with HIV (PLWH) on antiretroviral therapy. The median age was 57 (interquartile range, 52–63) years. PLWH exhibited a lower proportion of circulating CD14<sup>+</sup>CD16<sup>-</sup> classical monocytes (66.3% vs. 75.1%; <i>p</i> < 0.001), and higher proportion of CD14<sup>+</sup>CD16<sup>+</sup> inflammatory monocytes (17% vs. 11.7%; <i>p</i> = 0.005) compared to HIV-uninfected participants. PLWH had an increased expression of the chemokine receptor CX3CR1 in total monocytes (CX3CR1<sup>+</sup> monocytes, 24.5% vs. 4.7%; <i>p</i> < 0.001) and monocyte subsets. These findings were generally similar when analyzed by sex, with no significant interactions between sex and HIV status in adjusted models. Our data show that the inflammatory monocyte subset is expanded and monocyte CX3CR1 chemokine receptor expression is enhanced among PLWH, regardless of sex. Whether these parameters differentially affect risk for non-AIDS comorbidities and clinical outcomes in women with HIV requires additional investigation.Moises A. HuamanManuel G. FeriaCissy KityoSophie NalukwagoRashidah NazzindaDavid A. ZidarMarkella V. ZanniMark J. SiednerSteven K. GrinspoonChris T. LongeneckerMDPI AGarticlemonocytesinflammationactivationHIVwomensexMicrobiologyQR1-502ENViruses, Vol 13, Iss 2135, p 2135 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
monocytes inflammation activation HIV women sex Microbiology QR1-502 |
| spellingShingle |
monocytes inflammation activation HIV women sex Microbiology QR1-502 Moises A. Huaman Manuel G. Feria Cissy Kityo Sophie Nalukwago Rashidah Nazzinda David A. Zidar Markella V. Zanni Mark J. Siedner Steven K. Grinspoon Chris T. Longenecker A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| description |
Women with HIV may experience higher rates of non-AIDS comorbidities compared to men with HIV, but the underlying mechanisms are not well understood. We investigated sex-related differences in the effects of HIV on monocyte phenotypes within the Ugandan Study of HIV effects on the Myocardium and Atherosclerosis (mUTIMA). Of 133 participants who provided blood for flow cytometry assays, 86 (65%) were women and 91 (68%) were persons living with HIV (PLWH) on antiretroviral therapy. The median age was 57 (interquartile range, 52–63) years. PLWH exhibited a lower proportion of circulating CD14<sup>+</sup>CD16<sup>-</sup> classical monocytes (66.3% vs. 75.1%; <i>p</i> < 0.001), and higher proportion of CD14<sup>+</sup>CD16<sup>+</sup> inflammatory monocytes (17% vs. 11.7%; <i>p</i> = 0.005) compared to HIV-uninfected participants. PLWH had an increased expression of the chemokine receptor CX3CR1 in total monocytes (CX3CR1<sup>+</sup> monocytes, 24.5% vs. 4.7%; <i>p</i> < 0.001) and monocyte subsets. These findings were generally similar when analyzed by sex, with no significant interactions between sex and HIV status in adjusted models. Our data show that the inflammatory monocyte subset is expanded and monocyte CX3CR1 chemokine receptor expression is enhanced among PLWH, regardless of sex. Whether these parameters differentially affect risk for non-AIDS comorbidities and clinical outcomes in women with HIV requires additional investigation. |
| format |
article |
| author |
Moises A. Huaman Manuel G. Feria Cissy Kityo Sophie Nalukwago Rashidah Nazzinda David A. Zidar Markella V. Zanni Mark J. Siedner Steven K. Grinspoon Chris T. Longenecker |
| author_facet |
Moises A. Huaman Manuel G. Feria Cissy Kityo Sophie Nalukwago Rashidah Nazzinda David A. Zidar Markella V. Zanni Mark J. Siedner Steven K. Grinspoon Chris T. Longenecker |
| author_sort |
Moises A. Huaman |
| title |
A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| title_short |
A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| title_full |
A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| title_fullStr |
A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| title_full_unstemmed |
A Sex-Stratified Analysis of Monocyte Phenotypes Associated with HIV Infection in Uganda |
| title_sort |
sex-stratified analysis of monocyte phenotypes associated with hiv infection in uganda |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/1db156596af344d59b92edcb667ef9e7 |
| work_keys_str_mv |
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