A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes...

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Autores principales: Olivier G. de Jong, Daniel E. Murphy, Imre Mäger, Eduard Willms, Antonio Garcia-Guerra, Jerney J. Gitz-Francois, Juliet Lefferts, Dhanu Gupta, Sander C. Steenbeek, Jacco van Rheenen, Samir El Andaloussi, Raymond M. Schiffelers, Matthew J. A. Wood, Pieter Vader
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/1db2023ed1d94f558ace5b4d70bad252
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spelling oai:doaj.org-article:1db2023ed1d94f558ace5b4d70bad2522021-12-02T17:31:56ZA CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA10.1038/s41467-020-14977-82041-1723https://doaj.org/article/1db2023ed1d94f558ace5b4d70bad2522020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14977-8https://doaj.org/toc/2041-1723Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes not previously linked to EV-mediated RNA delivery.Olivier G. de JongDaniel E. MurphyImre MägerEduard WillmsAntonio Garcia-GuerraJerney J. Gitz-FrancoisJuliet LeffertsDhanu GuptaSander C. SteenbeekJacco van RheenenSamir El AndaloussiRaymond M. SchiffelersMatthew J. A. WoodPieter VaderNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Olivier G. de Jong
Daniel E. Murphy
Imre Mäger
Eduard Willms
Antonio Garcia-Guerra
Jerney J. Gitz-Francois
Juliet Lefferts
Dhanu Gupta
Sander C. Steenbeek
Jacco van Rheenen
Samir El Andaloussi
Raymond M. Schiffelers
Matthew J. A. Wood
Pieter Vader
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
description Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known. Here the authors develop a CRISPR-based reporting system to detect the transfer of guide RNAs and identify genes not previously linked to EV-mediated RNA delivery.
format article
author Olivier G. de Jong
Daniel E. Murphy
Imre Mäger
Eduard Willms
Antonio Garcia-Guerra
Jerney J. Gitz-Francois
Juliet Lefferts
Dhanu Gupta
Sander C. Steenbeek
Jacco van Rheenen
Samir El Andaloussi
Raymond M. Schiffelers
Matthew J. A. Wood
Pieter Vader
author_facet Olivier G. de Jong
Daniel E. Murphy
Imre Mäger
Eduard Willms
Antonio Garcia-Guerra
Jerney J. Gitz-Francois
Juliet Lefferts
Dhanu Gupta
Sander C. Steenbeek
Jacco van Rheenen
Samir El Andaloussi
Raymond M. Schiffelers
Matthew J. A. Wood
Pieter Vader
author_sort Olivier G. de Jong
title A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
title_short A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
title_full A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
title_fullStr A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
title_full_unstemmed A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA
title_sort crispr-cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of rna
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/1db2023ed1d94f558ace5b4d70bad252
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