A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology and identify the cell surface protease TMPRSS2 as a...

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Autores principales: Joep Beumer, Maarten H. Geurts, Mart M. Lamers, Jens Puschhof, Jingshu Zhang, Jelte van der Vaart, Anna Z. Mykytyn, Tim I. Breugem, Samra Riesebosch, Debby Schipper, Petra B. van den Doel, Wim de Lau, Cayetano Pleguezuelos-Manzano, Georg Busslinger, Bart L. Haagmans, Hans Clevers
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/1db487b66712463886d067b96ac47dba
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spelling oai:doaj.org-article:1db487b66712463886d067b96ac47dba2021-12-02T15:15:45ZA CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses10.1038/s41467-021-25729-72041-1723https://doaj.org/article/1db487b66712463886d067b96ac47dba2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25729-7https://doaj.org/toc/2041-1723Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology and identify the cell surface protease TMPRSS2 as a potential therapeutic target.Joep BeumerMaarten H. GeurtsMart M. LamersJens PuschhofJingshu ZhangJelte van der VaartAnna Z. MykytynTim I. BreugemSamra RieseboschDebby SchipperPetra B. van den DoelWim de LauCayetano Pleguezuelos-ManzanoGeorg BusslingerBart L. HaagmansHans CleversNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Joep Beumer
Maarten H. Geurts
Mart M. Lamers
Jens Puschhof
Jingshu Zhang
Jelte van der Vaart
Anna Z. Mykytyn
Tim I. Breugem
Samra Riesebosch
Debby Schipper
Petra B. van den Doel
Wim de Lau
Cayetano Pleguezuelos-Manzano
Georg Busslinger
Bart L. Haagmans
Hans Clevers
A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
description Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology and identify the cell surface protease TMPRSS2 as a potential therapeutic target.
format article
author Joep Beumer
Maarten H. Geurts
Mart M. Lamers
Jens Puschhof
Jingshu Zhang
Jelte van der Vaart
Anna Z. Mykytyn
Tim I. Breugem
Samra Riesebosch
Debby Schipper
Petra B. van den Doel
Wim de Lau
Cayetano Pleguezuelos-Manzano
Georg Busslinger
Bart L. Haagmans
Hans Clevers
author_facet Joep Beumer
Maarten H. Geurts
Mart M. Lamers
Jens Puschhof
Jingshu Zhang
Jelte van der Vaart
Anna Z. Mykytyn
Tim I. Breugem
Samra Riesebosch
Debby Schipper
Petra B. van den Doel
Wim de Lau
Cayetano Pleguezuelos-Manzano
Georg Busslinger
Bart L. Haagmans
Hans Clevers
author_sort Joep Beumer
title A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
title_short A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
title_full A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
title_fullStr A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
title_full_unstemmed A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
title_sort crispr/cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/1db487b66712463886d067b96ac47dba
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