A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse

Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luis Janssen, Felipe Marques de Almeida, Thais Amanda Silva Damasceno, Rodrigo de Paula Baptista, Georgios Joannis Pappas, Tatiana Amabile de Campos, Vicente de Paulo Martins
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Klebsiella pneumoniae antimicrobial resistance strain type MLST one health water scarcity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1dbc8e5d01104357a915553625bc3b7c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1dbc8e5d01104357a915553625bc3b7c
record_format	dspace
spelling	oai:doaj.org-article:1dbc8e5d01104357a915553625bc3b7c2021-11-30T18:26:21ZA Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse1664-302X10.3389/fmicb.2021.732324https://doaj.org/article/1dbc8e5d01104357a915553625bc3b7c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.732324/fullhttps://doaj.org/toc/1664-302XAntimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of resistance in and outside of clinical settings is of utter importance. A colony of multidrug resistant (MDR) bacteria identified as Klebsiella spp., by 16S rDNA amplicon sequencing, has been isolated from an urban lake in Brazil, during a drug-degrading bacterial prospection. Genomic analyses revealed the bacteria as Klebsiella pneumoniae species. Furthermore, the in silico Multilocus Sequence Typing (MLST) identified the genome as a new sequence type, ST5236. The search for antimicrobial resistance genes (ARGs) detected the presence of genes against beta-lactams, fosfomycin, acriflavine and efflux pumps, as well as genes for heavy metal resistance. Of particular note, an extended-spectrum beta-lactamase gene (blaCTX-M-15) has been detected in close proximity to siphoviridae genes, while a carbapenemase gene (KPC-2) has been found in an extrachromosomal contig, within a novel non-Tn4401 genetic element (NTEKPC). An extrachromosomal contig found in the V3 isolate is identical to a contig of a K. pneumoniae isolate from a nearby hospital, which indicates a putative gene flow from the hospital network into Paranoá lake. The discovery of a MDR isolate in this lake is worrisome, as the region has recently undergone periods of water scarcity causing the lake, which receives treated wastewater effluent, and is already used for recreational purposes, to be used as an environmental buffer for drinking water reuse. Altogether, our results indicate an underrepresentation of environmental K. pneumoniae among available genomes, which may hamper the understanding of the population dynamics of the species in the environment and its consequences in the spread of ARGs and virulence genes.Luis JanssenFelipe Marques de AlmeidaThais Amanda Silva DamascenoRodrigo de Paula BaptistaRodrigo de Paula BaptistaGeorgios Joannis PappasTatiana Amabile de CamposVicente de Paulo MartinsFrontiers Media S.A.articleKlebsiella pneumoniaeantimicrobial resistancestrain typeMLSTone healthwater scarcityMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae antimicrobial resistance strain type MLST one health water scarcity Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae antimicrobial resistance strain type MLST one health water scarcity Microbiology QR1-502 Luis Janssen Felipe Marques de Almeida Thais Amanda Silva Damasceno Rodrigo de Paula Baptista Rodrigo de Paula Baptista Georgios Joannis Pappas Tatiana Amabile de Campos Vicente de Paulo Martins A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
description	Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of resistance in and outside of clinical settings is of utter importance. A colony of multidrug resistant (MDR) bacteria identified as Klebsiella spp., by 16S rDNA amplicon sequencing, has been isolated from an urban lake in Brazil, during a drug-degrading bacterial prospection. Genomic analyses revealed the bacteria as Klebsiella pneumoniae species. Furthermore, the in silico Multilocus Sequence Typing (MLST) identified the genome as a new sequence type, ST5236. The search for antimicrobial resistance genes (ARGs) detected the presence of genes against beta-lactams, fosfomycin, acriflavine and efflux pumps, as well as genes for heavy metal resistance. Of particular note, an extended-spectrum beta-lactamase gene (blaCTX-M-15) has been detected in close proximity to siphoviridae genes, while a carbapenemase gene (KPC-2) has been found in an extrachromosomal contig, within a novel non-Tn4401 genetic element (NTEKPC). An extrachromosomal contig found in the V3 isolate is identical to a contig of a K. pneumoniae isolate from a nearby hospital, which indicates a putative gene flow from the hospital network into Paranoá lake. The discovery of a MDR isolate in this lake is worrisome, as the region has recently undergone periods of water scarcity causing the lake, which receives treated wastewater effluent, and is already used for recreational purposes, to be used as an environmental buffer for drinking water reuse. Altogether, our results indicate an underrepresentation of environmental K. pneumoniae among available genomes, which may hamper the understanding of the population dynamics of the species in the environment and its consequences in the spread of ARGs and virulence genes.
format	article
author	Luis Janssen Felipe Marques de Almeida Thais Amanda Silva Damasceno Rodrigo de Paula Baptista Rodrigo de Paula Baptista Georgios Joannis Pappas Tatiana Amabile de Campos Vicente de Paulo Martins
author_facet	Luis Janssen Felipe Marques de Almeida Thais Amanda Silva Damasceno Rodrigo de Paula Baptista Rodrigo de Paula Baptista Georgios Joannis Pappas Tatiana Amabile de Campos Vicente de Paulo Martins
author_sort	Luis Janssen
title	A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
title_short	A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
title_full	A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
title_fullStr	A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
title_full_unstemmed	A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse
title_sort	novel multidrug resistant, non-tn4401 genetic element-bearing, strain of klebsiella pneumoniae isolated from an urban lake with drinking and recreational water reuse
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1dbc8e5d01104357a915553625bc3b7c
work_keys_str_mv	AT luisjanssen anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT felipemarquesdealmeida anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT thaisamandasilvadamasceno anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT rodrigodepaulabaptista anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT rodrigodepaulabaptista anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT georgiosjoannispappas anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT tatianaamabiledecampos anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT vicentedepaulomartins anovelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT luisjanssen novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT felipemarquesdealmeida novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT thaisamandasilvadamasceno novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT rodrigodepaulabaptista novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT rodrigodepaulabaptista novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT georgiosjoannispappas novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT tatianaamabiledecampos novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse AT vicentedepaulomartins novelmultidrugresistantnontn4401geneticelementbearingstrainofklebsiellapneumoniaeisolatedfromanurbanlakewithdrinkingandrecreationalwaterreuse
_version_	1718406365762813952

A Novel Multidrug Resistant, Non-Tn4401 Genetic Element-Bearing, Strain of Klebsiella pneumoniae Isolated From an Urban Lake With Drinking and Recreational Water Reuse

Ejemplares similares