Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration

Abstract Background The poor regenerative capability and structural complexity make the reconstruction of meniscus particularly challenging in clinic. 3D printing of polymer scaffolds holds the promise of precisely constructing complex tissue architecture, however the resultant scaffolds usually lac...

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Autores principales:	Xingyu Deng, Xiabin Chen, Fang Geng, Xin Tang, Zhenzhen Li, Jie Zhang, Yikai Wang, Fangqian Wang, Na Zheng, Peng Wang, Xiaohua Yu, Shurong Hou, Wei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Meniscus Tissue engineering Scaffold Chondrogenic differentiation Biotechnology TP248.13-248.65 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/1dbdf370d90b4332bfc0fb2d244bdb13
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spelling	oai:doaj.org-article:1dbdf370d90b4332bfc0fb2d244bdb132021-12-05T12:19:06ZPrecision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration10.1186/s12951-021-01141-71477-3155https://doaj.org/article/1dbdf370d90b4332bfc0fb2d244bdb132021-12-01T00:00:00Zhttps://doi.org/10.1186/s12951-021-01141-7https://doaj.org/toc/1477-3155Abstract Background The poor regenerative capability and structural complexity make the reconstruction of meniscus particularly challenging in clinic. 3D printing of polymer scaffolds holds the promise of precisely constructing complex tissue architecture, however the resultant scaffolds usually lack of sufficient bioactivity to effectively generate new tissue. Results Herein, 3D printing-based strategy via the cryo-printing technology was employed to fabricate customized polyurethane (PU) porous scaffolds that mimic native meniscus. In order to enhance scaffold bioactivity for human mesenchymal stem cells (hMSCs) culture, scaffold surface modification through the physical absorption of collagen I and fibronectin (FN) were investigated by cell live/dead staining and cell viability assays. The results indicated that coating with fibronectin outperformed coating with collagen I in promoting multiple-aspect stem cell functions, and fibronectin favors long-term culture required for chondrogenesis on scaffolds. In situ chondrogenic differentiation of hMSCs resulted in a time-dependent upregulation of SOX9 and extracellular matrix (ECM) assessed by qRT-PCR analysis, and enhanced deposition of collagen II and aggrecan confirmed by immunostaining and western blot analysis. Gene expression data also revealed 3D porous scaffolds coupled with surface functionalization greatly facilitated chondrogenesis of hMSCs. In addition, the subcutaneous implantation of 3D porous PU scaffolds on SD rats did not induce local inflammation and integrated well with surrounding tissues, suggesting good in vivo biocompatibility. Conclusions Overall, this study presents an approach to fabricate biocompatible meniscus constructs that not only recapitulate the architecture and mechanical property of native meniscus, but also have desired bioactivity for hMSCs culture and cartilage regeneration. The generated 3D meniscus-mimicking scaffolds incorporated with hMSCs offer great promise in tissue engineering strategies for meniscus regeneration. Graphical AbstractXingyu DengXiabin ChenFang GengXin TangZhenzhen LiJie ZhangYikai WangFangqian WangNa ZhengPeng WangXiaohua YuShurong HouWei ZhangBMCarticleMeniscusTissue engineeringScaffoldChondrogenic differentiationBiotechnologyTP248.13-248.65Medical technologyR855-855.5ENJournal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Meniscus Tissue engineering Scaffold Chondrogenic differentiation Biotechnology TP248.13-248.65 Medical technology R855-855.5
spellingShingle	Meniscus Tissue engineering Scaffold Chondrogenic differentiation Biotechnology TP248.13-248.65 Medical technology R855-855.5 Xingyu Deng Xiabin Chen Fang Geng Xin Tang Zhenzhen Li Jie Zhang Yikai Wang Fangqian Wang Na Zheng Peng Wang Xiaohua Yu Shurong Hou Wei Zhang Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
description	Abstract Background The poor regenerative capability and structural complexity make the reconstruction of meniscus particularly challenging in clinic. 3D printing of polymer scaffolds holds the promise of precisely constructing complex tissue architecture, however the resultant scaffolds usually lack of sufficient bioactivity to effectively generate new tissue. Results Herein, 3D printing-based strategy via the cryo-printing technology was employed to fabricate customized polyurethane (PU) porous scaffolds that mimic native meniscus. In order to enhance scaffold bioactivity for human mesenchymal stem cells (hMSCs) culture, scaffold surface modification through the physical absorption of collagen I and fibronectin (FN) were investigated by cell live/dead staining and cell viability assays. The results indicated that coating with fibronectin outperformed coating with collagen I in promoting multiple-aspect stem cell functions, and fibronectin favors long-term culture required for chondrogenesis on scaffolds. In situ chondrogenic differentiation of hMSCs resulted in a time-dependent upregulation of SOX9 and extracellular matrix (ECM) assessed by qRT-PCR analysis, and enhanced deposition of collagen II and aggrecan confirmed by immunostaining and western blot analysis. Gene expression data also revealed 3D porous scaffolds coupled with surface functionalization greatly facilitated chondrogenesis of hMSCs. In addition, the subcutaneous implantation of 3D porous PU scaffolds on SD rats did not induce local inflammation and integrated well with surrounding tissues, suggesting good in vivo biocompatibility. Conclusions Overall, this study presents an approach to fabricate biocompatible meniscus constructs that not only recapitulate the architecture and mechanical property of native meniscus, but also have desired bioactivity for hMSCs culture and cartilage regeneration. The generated 3D meniscus-mimicking scaffolds incorporated with hMSCs offer great promise in tissue engineering strategies for meniscus regeneration. Graphical Abstract
format	article
author	Xingyu Deng Xiabin Chen Fang Geng Xin Tang Zhenzhen Li Jie Zhang Yikai Wang Fangqian Wang Na Zheng Peng Wang Xiaohua Yu Shurong Hou Wei Zhang
author_facet	Xingyu Deng Xiabin Chen Fang Geng Xin Tang Zhenzhen Li Jie Zhang Yikai Wang Fangqian Wang Na Zheng Peng Wang Xiaohua Yu Shurong Hou Wei Zhang
author_sort	Xingyu Deng
title	Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
title_short	Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
title_full	Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
title_fullStr	Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
title_full_unstemmed	Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration
title_sort	precision 3d printed meniscus scaffolds to facilitate hmscs proliferation and chondrogenic differentiation for tissue regeneration
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1dbdf370d90b4332bfc0fb2d244bdb13
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_version_	1718372003692412928

Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration

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