Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan

Abstract Background Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals. Methods In the present study, we detected IgG and IgM antibodies through an E...

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Autores principales:	Abdul Majid, Nisar Ahmad, Sumbal Haleem, Noor ul Akbar, Shehzad Zareen, Muhammad Taib, Sanaullah Khan, Rafiq Hussain, Sohail
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	T. gondii Seroprevalence Toxoplasmosis Cats Khyber Pakhtunkhwa, ELISA, PCR Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/1dbf2908317848a8860fa887f38260c9
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spelling	oai:doaj.org-article:1dbf2908317848a8860fa887f38260c92021-11-21T12:25:50ZDetection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan10.1186/s12917-021-03064-91746-6148https://doaj.org/article/1dbf2908317848a8860fa887f38260c92021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03064-9https://doaj.org/toc/1746-6148Abstract Background Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals. Methods In the present study, we detected IgG and IgM antibodies through an ELISA kit and DNA of T. gondii through PCR in 197 pets and stray cats in Peshawar, Charsadda, Mardan, and Kohat districts of Khyber Pakhtunkhwa (Pakistan) to estimate the existence of feline toxoplasmosis. Results The current study revealed that stray cats have a significant infection rate of T. gondii (74.6%) as compared to pet cats (25.4%). In all the four districts, the prevalence of T. gondii was pointedly higher in district Kohat (95.5%) in the feline population. In comparison to the female (75.18%) and male (both pets and stray) cats have a maximum infection of (81.66%) non-significantly. The prevalence of T. gondii was observed to be significantly higher (91.66%) in the older and greater than 4 year old population of cats as compared to the younger ones. In poor health condition, the cat populations has a higher risk of infection of 92.3% as compared to healthy and poor body condition (73.91%) and (82.6%) respectively. The chronic and reactivated chronic conditions of toxoplasmosis were higher (58.37%) as compared to the acute condition. Conclusion It has been concluded that toxoplasmosis is widely spread in the studied population.The outcomes of the present study show that T. gondii infection has a significant impact on the type of cat, age, and area, which implies a serious threat to human beings. Therefore, genotyping of T. gondii strains from different hosts is needed to forecast the current approach for prevention and control of this zoonotic parasite.Abdul MajidNisar AhmadSumbal HaleemNoor ul AkbarShehzad ZareenMuhammad TaibSanaullah KhanRafiq HussainSohailBMCarticleT. gondiiSeroprevalenceToxoplasmosisCatsKhyber Pakhtunkhwa, ELISA, PCRVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	T. gondii Seroprevalence Toxoplasmosis Cats Khyber Pakhtunkhwa, ELISA, PCR Veterinary medicine SF600-1100
spellingShingle	T. gondii Seroprevalence Toxoplasmosis Cats Khyber Pakhtunkhwa, ELISA, PCR Veterinary medicine SF600-1100 Abdul Majid Nisar Ahmad Sumbal Haleem Noor ul Akbar Shehzad Zareen Muhammad Taib Sanaullah Khan Rafiq Hussain Sohail Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
description	Abstract Background Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals. Methods In the present study, we detected IgG and IgM antibodies through an ELISA kit and DNA of T. gondii through PCR in 197 pets and stray cats in Peshawar, Charsadda, Mardan, and Kohat districts of Khyber Pakhtunkhwa (Pakistan) to estimate the existence of feline toxoplasmosis. Results The current study revealed that stray cats have a significant infection rate of T. gondii (74.6%) as compared to pet cats (25.4%). In all the four districts, the prevalence of T. gondii was pointedly higher in district Kohat (95.5%) in the feline population. In comparison to the female (75.18%) and male (both pets and stray) cats have a maximum infection of (81.66%) non-significantly. The prevalence of T. gondii was observed to be significantly higher (91.66%) in the older and greater than 4 year old population of cats as compared to the younger ones. In poor health condition, the cat populations has a higher risk of infection of 92.3% as compared to healthy and poor body condition (73.91%) and (82.6%) respectively. The chronic and reactivated chronic conditions of toxoplasmosis were higher (58.37%) as compared to the acute condition. Conclusion It has been concluded that toxoplasmosis is widely spread in the studied population.The outcomes of the present study show that T. gondii infection has a significant impact on the type of cat, age, and area, which implies a serious threat to human beings. Therefore, genotyping of T. gondii strains from different hosts is needed to forecast the current approach for prevention and control of this zoonotic parasite.
format	article
author	Abdul Majid Nisar Ahmad Sumbal Haleem Noor ul Akbar Shehzad Zareen Muhammad Taib Sanaullah Khan Rafiq Hussain Sohail
author_facet	Abdul Majid Nisar Ahmad Sumbal Haleem Noor ul Akbar Shehzad Zareen Muhammad Taib Sanaullah Khan Rafiq Hussain Sohail
author_sort	Abdul Majid
title	Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
title_short	Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
title_full	Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
title_fullStr	Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
title_full_unstemmed	Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
title_sort	detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in khyber pakhtunkhwa, pakistan
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1dbf2908317848a8860fa887f38260c9
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Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan

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