Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway

Objective: To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism. Methods: C57BL/6 mice were randomly divided into Ctrl group (normal control group), DM group (STZ intraperitoneal injection model), and DM+ICA group (diabetic C57BL/6 mice by intrag...

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Autores principales:	Bing Wu, Feng Guo
Formato:	article
Lenguaje:	EN
Publicado:	Editorial Board of Journal of Hainan Medical University 2021
Materias:	icariin ampk mtor autophagy diabetic myocardial hypertrophy Medicine R
Acceso en línea:	https://doaj.org/article/1dc3e47c37114bb2ad0889e65525a1a3
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spelling	oai:doaj.org-article:1dc3e47c37114bb2ad0889e65525a1a32021-12-01T05:11:28ZIcariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway1007-1237https://doaj.org/article/1dc3e47c37114bb2ad0889e65525a1a32021-10-01T00:00:00Zhttp://www.hnykdxxb.com/PDF/202120/05.pdfhttps://doaj.org/toc/1007-1237Objective: To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism. Methods: C57BL/6 mice were randomly divided into Ctrl group (normal control group), DM group (STZ intraperitoneal injection model), and DM+ICA group (diabetic C57BL/6 mice by intragastric Icariin solution 80mg/kg/d, for 3 consecutive weeks). Real-time quantitative PCR was used to detect myocardial hypertrophy markers BNP and β-MHC. Western blotting was used to detect myocardial AMPK, p-AMPK, mTOR, p-mTOR, LC3B and Beclin1 protein expression. Echocardiogram was used to detect left ventricular mass and ejection fraction. Results: Compared with the normal control group, the expression of myocardial hypertrophy markers BNP and β-MHC mRNA in diabetic mice were significantly increased; the expression of phosphorylated AMPK protein, autophagy-related protein LC3B and Beclin1 were significantly increased, and the expression of phosphorylated mTOR protein is significantly reduced; the left ventricular mass is significantly increased. The above changes can be reversed after treatment with Icariin, but the effect of Icariin is blocked by the autophagy inhibitor rapamycin. Conclusion: Icariin may inhibit autophagy and reduce diabetic myocardial hypertrophy through AMPK-mTOR signaling pathway.Bing WuFeng GuoEditorial Board of Journal of Hainan Medical Universityarticleicariinampkmtorautophagydiabetic myocardial hypertrophyMedicineRENJournal of Hainan Medical University, Vol 27, Iss 20, Pp 20-24 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	icariin ampk mtor autophagy diabetic myocardial hypertrophy Medicine R
spellingShingle	icariin ampk mtor autophagy diabetic myocardial hypertrophy Medicine R Bing Wu Feng Guo Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
description	Objective: To observe the effect of Icariin on diabetic myocardial hypertrophy and explore its molecular mechanism. Methods: C57BL/6 mice were randomly divided into Ctrl group (normal control group), DM group (STZ intraperitoneal injection model), and DM+ICA group (diabetic C57BL/6 mice by intragastric Icariin solution 80mg/kg/d, for 3 consecutive weeks). Real-time quantitative PCR was used to detect myocardial hypertrophy markers BNP and β-MHC. Western blotting was used to detect myocardial AMPK, p-AMPK, mTOR, p-mTOR, LC3B and Beclin1 protein expression. Echocardiogram was used to detect left ventricular mass and ejection fraction. Results: Compared with the normal control group, the expression of myocardial hypertrophy markers BNP and β-MHC mRNA in diabetic mice were significantly increased; the expression of phosphorylated AMPK protein, autophagy-related protein LC3B and Beclin1 were significantly increased, and the expression of phosphorylated mTOR protein is significantly reduced; the left ventricular mass is significantly increased. The above changes can be reversed after treatment with Icariin, but the effect of Icariin is blocked by the autophagy inhibitor rapamycin. Conclusion: Icariin may inhibit autophagy and reduce diabetic myocardial hypertrophy through AMPK-mTOR signaling pathway.
format	article
author	Bing Wu Feng Guo
author_facet	Bing Wu Feng Guo
author_sort	Bing Wu
title	Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
title_short	Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
title_full	Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
title_fullStr	Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
title_full_unstemmed	Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway
title_sort	icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the ampk/mtor pathway
publisher	Editorial Board of Journal of Hainan Medical University
publishDate	2021
url	https://doaj.org/article/1dc3e47c37114bb2ad0889e65525a1a3
work_keys_str_mv	AT bingwu icariinamelioratediabeticmyocardialhypertrophybyinhibitingautophagyviatheampkmtorpathway AT fengguo icariinamelioratediabeticmyocardialhypertrophybyinhibitingautophagyviatheampkmtorpathway
_version_	1718405527537451008

Icariin ameliorate diabetic myocardial hypertrophy by inhibiting autophagy via the AMPK/mTOR pathway

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