Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis

CTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP an...

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Autores principales:	Leo Bellin, Vanessa Scherer, Eva Dörfer, Anne Lau, Alexandre Magno Vicente, Jörg Meurer, Daniel Hickl, Torsten Möhlmann
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	pyrimidine de novo synthesis CTP photosynthesis chloroplast genome deoxycytidine Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/1dc81fe146a74a82a3c69984215f8f29
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spelling	oai:doaj.org-article:1dc81fe146a74a82a3c69984215f8f292021-12-01T16:35:02ZCytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis1664-462X10.3389/fpls.2021.789189https://doaj.org/article/1dc81fe146a74a82a3c69984215f8f292021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.789189/fullhttps://doaj.org/toc/1664-462XCTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP and its deoxy form dCTP are present at low levels in developing seedlings. Thus, under conditions of fast proliferation, the synthesis of CTP (dCTP) can become a limiting factor for RNA and DNA synthesis. The higher sensitivity of ami-CTPS2 lines toward the DNA-Gyrase inhibitor ciprofloxacin, together with reduced plastid DNA copy number and 16S and 23S chloroplast ribosomal RNA support this view. High expression and proposed beneficial biochemical features render CTPS2 the most important isoform for early seedling development. In addition, CTPS2 was identified as an essential enzyme in embryo development before, as knock-out mutants were embryo lethal. In line with this, ami-CTPS2 lines also exhibited reduced seed numbers per plant.Leo BellinVanessa SchererEva DörferAnne LauAlexandre Magno VicenteJörg MeurerDaniel HicklTorsten MöhlmannFrontiers Media S.A.articlepyrimidine de novo synthesisCTPphotosynthesischloroplastgenomedeoxycytidinePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pyrimidine de novo synthesis CTP photosynthesis chloroplast genome deoxycytidine Plant culture SB1-1110
spellingShingle	pyrimidine de novo synthesis CTP photosynthesis chloroplast genome deoxycytidine Plant culture SB1-1110 Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
description	CTP synthases (CTPS) comprise a protein family of the five members CTPS1-CTPS5 in Arabidopsis, all located in the cytosol. Specifically, downregulation of CTPS2 by amiRNA technology results in plants with defects in chlorophyll accumulation and photosynthetic performance early in development. CTP and its deoxy form dCTP are present at low levels in developing seedlings. Thus, under conditions of fast proliferation, the synthesis of CTP (dCTP) can become a limiting factor for RNA and DNA synthesis. The higher sensitivity of ami-CTPS2 lines toward the DNA-Gyrase inhibitor ciprofloxacin, together with reduced plastid DNA copy number and 16S and 23S chloroplast ribosomal RNA support this view. High expression and proposed beneficial biochemical features render CTPS2 the most important isoform for early seedling development. In addition, CTPS2 was identified as an essential enzyme in embryo development before, as knock-out mutants were embryo lethal. In line with this, ami-CTPS2 lines also exhibited reduced seed numbers per plant.
format	article
author	Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann
author_facet	Leo Bellin Vanessa Scherer Eva Dörfer Anne Lau Alexandre Magno Vicente Jörg Meurer Daniel Hickl Torsten Möhlmann
author_sort	Leo Bellin
title	Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
title_short	Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
title_full	Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
title_fullStr	Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
title_full_unstemmed	Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis
title_sort	cytosolic ctp production limits the establishment of photosynthesis in arabidopsis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1dc81fe146a74a82a3c69984215f8f29
work_keys_str_mv	AT leobellin cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT vanessascherer cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT evadorfer cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT annelau cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT alexandremagnovicente cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT jorgmeurer cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT danielhickl cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis AT torstenmohlmann cytosolicctpproductionlimitstheestablishmentofphotosynthesisinarabidopsis
_version_	1718404737945042944

Cytosolic CTP Production Limits the Establishment of Photosynthesis in Arabidopsis

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