Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes

Spatial transcriptomics aims to pair omic data with tissue structure. Here the authors report Spatially PhotoActivatable Colour Encoded Cell Address Tags (SPACECAT) to track and isolate live cells by location; this enables spatially informed downstream assays like scRNA-seq and flow cytometry.

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Autores principales:	Alex S Genshaft, Carly G. K. Ziegler, Constantine N. Tzouanas, Benjamin E. Mead, Alex M. Jaeger, Andrew W. Navia, Ryan P. King, Miyeko D. Mana, Siyi Huang, Vanessa Mitsialis, Scott B. Snapper, Ömer H. Yilmaz, Tyler Jacks, Jeffrey F. Van Humbeck, Alex K. Shalek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1dca5ab3f8864e5791c305a732202ce2
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spelling	oai:doaj.org-article:1dca5ab3f8864e5791c305a732202ce22021-12-02T15:10:55ZLive cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes10.1038/s41467-021-25279-y2041-1723https://doaj.org/article/1dca5ab3f8864e5791c305a732202ce22021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25279-yhttps://doaj.org/toc/2041-1723Spatial transcriptomics aims to pair omic data with tissue structure. Here the authors report Spatially PhotoActivatable Colour Encoded Cell Address Tags (SPACECAT) to track and isolate live cells by location; this enables spatially informed downstream assays like scRNA-seq and flow cytometry.Alex S GenshaftCarly G. K. ZieglerConstantine N. TzouanasBenjamin E. MeadAlex M. JaegerAndrew W. NaviaRyan P. KingMiyeko D. ManaSiyi HuangVanessa MitsialisScott B. SnapperÖmer H. YilmazTyler JacksJeffrey F. Van HumbeckAlex K. ShalekNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alex S Genshaft Carly G. K. Ziegler Constantine N. Tzouanas Benjamin E. Mead Alex M. Jaeger Andrew W. Navia Ryan P. King Miyeko D. Mana Siyi Huang Vanessa Mitsialis Scott B. Snapper Ömer H. Yilmaz Tyler Jacks Jeffrey F. Van Humbeck Alex K. Shalek Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
description	Spatial transcriptomics aims to pair omic data with tissue structure. Here the authors report Spatially PhotoActivatable Colour Encoded Cell Address Tags (SPACECAT) to track and isolate live cells by location; this enables spatially informed downstream assays like scRNA-seq and flow cytometry.
format	article
author	Alex S Genshaft Carly G. K. Ziegler Constantine N. Tzouanas Benjamin E. Mead Alex M. Jaeger Andrew W. Navia Ryan P. King Miyeko D. Mana Siyi Huang Vanessa Mitsialis Scott B. Snapper Ömer H. Yilmaz Tyler Jacks Jeffrey F. Van Humbeck Alex K. Shalek
author_facet	Alex S Genshaft Carly G. K. Ziegler Constantine N. Tzouanas Benjamin E. Mead Alex M. Jaeger Andrew W. Navia Ryan P. King Miyeko D. Mana Siyi Huang Vanessa Mitsialis Scott B. Snapper Ömer H. Yilmaz Tyler Jacks Jeffrey F. Van Humbeck Alex K. Shalek
author_sort	Alex S Genshaft
title	Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
title_short	Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
title_full	Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
title_fullStr	Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
title_full_unstemmed	Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
title_sort	live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1dca5ab3f8864e5791c305a732202ce2
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Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes

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