The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.

This work shows that the recently described Escherichia coli BtuE peroxidase protects the bacterium against oxidative stress that is generated by tellurite and by other reactive oxygen species elicitors (ROS). Cells lacking btuE (ΔbtuE) displayed higher sensitivity to K(2)TeO(3) and other oxidative...

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Autores principales:	Felipe A Arenas, Paulo C Covarrubias, Juan M Sandoval, José M Pérez-Donoso, James A Imlay, Claudio C Vásquez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1dcc5dbde9d9428ea0991a83170cacb2
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spelling	oai:doaj.org-article:1dcc5dbde9d9428ea0991a83170cacb22021-11-18T07:00:37ZThe Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.1932-620310.1371/journal.pone.0015979https://doaj.org/article/1dcc5dbde9d9428ea0991a83170cacb22011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21264338/?tool=EBIhttps://doaj.org/toc/1932-6203This work shows that the recently described Escherichia coli BtuE peroxidase protects the bacterium against oxidative stress that is generated by tellurite and by other reactive oxygen species elicitors (ROS). Cells lacking btuE (ΔbtuE) displayed higher sensitivity to K(2)TeO(3) and other oxidative stress-generating agents than did the isogenic, parental, wild-type strain. They also exhibited increased levels of cytoplasmic reactive oxygen species, oxidized proteins, thiobarbituric acid reactive substances, and lipoperoxides. E. coli ΔbtuE that was exposed to tellurite or H(2)O(2) did not show growth changes relative to wild type cells either in aerobic or anaerobic conditions. Nevertheless, the elimination of btuE from cells deficient in catalases/peroxidases (Hpx(-)) resulted in impaired growth and resistance to these toxicants only in aerobic conditions, suggesting that BtuE is involved in the defense against oxidative damage. Genetic complementation of E. coli ΔbtuE restored toxicant resistance to levels exhibited by the wild type strain. As expected, btuE overexpression resulted in decreased amounts of oxidative damage products as well as in lower transcriptional levels of the oxidative stress-induced genes ibpA, soxS and katG.Felipe A ArenasPaulo C CovarrubiasJuan M SandovalJosé M Pérez-DonosoJames A ImlayClaudio C VásquezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15979 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Felipe A Arenas Paulo C Covarrubias Juan M Sandoval José M Pérez-Donoso James A Imlay Claudio C Vásquez The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
description	This work shows that the recently described Escherichia coli BtuE peroxidase protects the bacterium against oxidative stress that is generated by tellurite and by other reactive oxygen species elicitors (ROS). Cells lacking btuE (ΔbtuE) displayed higher sensitivity to K(2)TeO(3) and other oxidative stress-generating agents than did the isogenic, parental, wild-type strain. They also exhibited increased levels of cytoplasmic reactive oxygen species, oxidized proteins, thiobarbituric acid reactive substances, and lipoperoxides. E. coli ΔbtuE that was exposed to tellurite or H(2)O(2) did not show growth changes relative to wild type cells either in aerobic or anaerobic conditions. Nevertheless, the elimination of btuE from cells deficient in catalases/peroxidases (Hpx(-)) resulted in impaired growth and resistance to these toxicants only in aerobic conditions, suggesting that BtuE is involved in the defense against oxidative damage. Genetic complementation of E. coli ΔbtuE restored toxicant resistance to levels exhibited by the wild type strain. As expected, btuE overexpression resulted in decreased amounts of oxidative damage products as well as in lower transcriptional levels of the oxidative stress-induced genes ibpA, soxS and katG.
format	article
author	Felipe A Arenas Paulo C Covarrubias Juan M Sandoval José M Pérez-Donoso James A Imlay Claudio C Vásquez
author_facet	Felipe A Arenas Paulo C Covarrubias Juan M Sandoval José M Pérez-Donoso James A Imlay Claudio C Vásquez
author_sort	Felipe A Arenas
title	The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
title_short	The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
title_full	The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
title_fullStr	The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
title_full_unstemmed	The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.
title_sort	escherichia coli btue protein functions as a resistance determinant against reactive oxygen species.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1dcc5dbde9d9428ea0991a83170cacb2
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The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species.

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