Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis

Currently, one of the topical areas of research is the development of new antigen preparations for the specific diagnosis and prevention of brucellosis, since indication of the pathogen and prevention of the disease is complicated by the ability of brucella to dissociate, and live vaccines used for...

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Autores principales:	V. I. Dubrovina, O. V. Yuryeva, A. B. Pyatidesyatnikova, T. P. Starovoytova, Zh. A. Konovalova, N. L. Barannikova, V. B. Nikolayev, S. V. Balakhonov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	brucellosis antigens immunity phagocytes Science Q
Acceso en línea:	https://doaj.org/article/1dcfbdf5a3ce410a9f87ed463558e4b5
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spelling	oai:doaj.org-article:1dcfbdf5a3ce410a9f87ed463558e4b52021-11-23T06:14:42ZProspects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis2541-94202587-959610.29413/ABS.2019-4.3.12https://doaj.org/article/1dcfbdf5a3ce410a9f87ed463558e4b52019-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2090https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Currently, one of the topical areas of research is the development of new antigen preparations for the specific diagnosis and prevention of brucellosis, since indication of the pathogen and prevention of the disease is complicated by the ability of brucella to dissociate, and live vaccines used for specific prevention of brucellosis have residual virulence. Thermal extracts (TE) obtained from Brucella abortus I-206 in the L- and S-form can be used as such promising antigens. It is known that TE in the L- and S-forms have immunogenic properties, as well as a modulating effect on the proliferation of immunocompetent cells, morphological changes in the immunocompetent organs of experimental animals.The aimof the work is to study the effect of Brucella abortus thermal extracts in L- and S-forms on the functional state of the cells of experimental animals.Materials and methods. The study was performed on 100 certified white mice. As objects of study, we used the B. abortus I-206 TE in L- and S-forms. Evaluation of the effect of antigenic drugs on the functional state of phagocytes of laboratory animals in vitro was performed on peritoneal macrophages. The total activity of the respiratory chain enzymes in the NBT-test and superoxide dismutase was determined. Cells of intact animals served as controls. As a positive control, a commercial antigenic LPS preparation Escherichia coli was used. The content of cyclic nucleotides in homogenates of immunocompetent organs was determined using ELISA.Results.This study presents materials on the study of the effect of TE on the bactericidal activity of phagocytes and the level of cyclic nucleotides in immunocompetent organs. It has been established that TEs activate oxygen-dependent bactericidal systems of phagocytes. When studying the effect of TE on the content of cyclic nucleotides in immunocompetent organs of white mice, an increase in their concentration was revealed, indicating an increase in the functional activity of the cells.Conclusion.The obtained data make it possible to substantiate the need for a further detailed study of the immunogenic properties of B. abortus TE in the L- or S-form on the organism of experimental animals.V. I. DubrovinaO. V. YuryevaA. B. PyatidesyatnikovaT. P. StarovoytovaZh. A. KonovalovaN. L. BarannikovaV. B. NikolayevS. V. BalakhonovScientific Сentre for Family Health and Human Reproduction ProblemsarticlebrucellosisantigensimmunityphagocytesScienceQRUActa Biomedica Scientifica, Vol 4, Iss 3, Pp 96-101 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	brucellosis antigens immunity phagocytes Science Q
spellingShingle	brucellosis antigens immunity phagocytes Science Q V. I. Dubrovina O. V. Yuryeva A. B. Pyatidesyatnikova T. P. Starovoytova Zh. A. Konovalova N. L. Barannikova V. B. Nikolayev S. V. Balakhonov Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
description	Currently, one of the topical areas of research is the development of new antigen preparations for the specific diagnosis and prevention of brucellosis, since indication of the pathogen and prevention of the disease is complicated by the ability of brucella to dissociate, and live vaccines used for specific prevention of brucellosis have residual virulence. Thermal extracts (TE) obtained from Brucella abortus I-206 in the L- and S-form can be used as such promising antigens. It is known that TE in the L- and S-forms have immunogenic properties, as well as a modulating effect on the proliferation of immunocompetent cells, morphological changes in the immunocompetent organs of experimental animals.The aimof the work is to study the effect of Brucella abortus thermal extracts in L- and S-forms on the functional state of the cells of experimental animals.Materials and methods. The study was performed on 100 certified white mice. As objects of study, we used the B. abortus I-206 TE in L- and S-forms. Evaluation of the effect of antigenic drugs on the functional state of phagocytes of laboratory animals in vitro was performed on peritoneal macrophages. The total activity of the respiratory chain enzymes in the NBT-test and superoxide dismutase was determined. Cells of intact animals served as controls. As a positive control, a commercial antigenic LPS preparation Escherichia coli was used. The content of cyclic nucleotides in homogenates of immunocompetent organs was determined using ELISA.Results.This study presents materials on the study of the effect of TE on the bactericidal activity of phagocytes and the level of cyclic nucleotides in immunocompetent organs. It has been established that TEs activate oxygen-dependent bactericidal systems of phagocytes. When studying the effect of TE on the content of cyclic nucleotides in immunocompetent organs of white mice, an increase in their concentration was revealed, indicating an increase in the functional activity of the cells.Conclusion.The obtained data make it possible to substantiate the need for a further detailed study of the immunogenic properties of B. abortus TE in the L- or S-form on the organism of experimental animals.
format	article
author	V. I. Dubrovina O. V. Yuryeva A. B. Pyatidesyatnikova T. P. Starovoytova Zh. A. Konovalova N. L. Barannikova V. B. Nikolayev S. V. Balakhonov
author_facet	V. I. Dubrovina O. V. Yuryeva A. B. Pyatidesyatnikova T. P. Starovoytova Zh. A. Konovalova N. L. Barannikova V. B. Nikolayev S. V. Balakhonov
author_sort	V. I. Dubrovina
title	Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
title_short	Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
title_full	Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
title_fullStr	Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
title_full_unstemmed	Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis
title_sort	prospects for the use of thermal extracts of brucella abortusi-206 in s-and l-forms in the diagnosis and prevention of brucellosis
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/1dcfbdf5a3ce410a9f87ed463558e4b5
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Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis

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