Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

Abstract The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal de...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karl J. Wahlin, Julien A. Maruotti, Srinivasa R. Sripathi, John Ball, Juan M. Angueyra, Catherine Kim, Rhonda Grebe, Wei Li, Bryan W. Jones, Donald J. Zack
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1dd261a3fd8c4e21bc9180331f614bf5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1dd261a3fd8c4e21bc9180331f614bf5
record_format	dspace
spelling	oai:doaj.org-article:1dd261a3fd8c4e21bc9180331f614bf52021-12-02T15:06:25ZPhotoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells10.1038/s41598-017-00774-92045-2322https://doaj.org/article/1dd261a3fd8c4e21bc9180331f614bf52017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00774-9https://doaj.org/toc/2045-2322Abstract The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal development, provide a platform to investigate the mechanisms of retinal degeneration and screen for neuroprotective compounds, and provide the basis for cell-based therapeutic strategies. In this study, we describe an in vitro method by which hPSCs can be differentiated into 3D retinas with at least some important features reminiscent of a mature retina, including exuberant outgrowth of outer segment-like structures and synaptic ribbons, photoreceptor neurotransmitter expression, and membrane conductances and synaptic vesicle release properties consistent with possible photoreceptor synaptic function. The advanced outer segment-like structures reported here support the notion that 3D retina cups could serve as a model for studying mature photoreceptor development and allow for more robust modeling of retinal degenerative disease in vitro.Karl J. WahlinJulien A. MaruottiSrinivasa R. SripathiJohn BallJuan M. AngueyraCatherine KimRhonda GrebeWei LiBryan W. JonesDonald J. ZackNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karl J. Wahlin Julien A. Maruotti Srinivasa R. Sripathi John Ball Juan M. Angueyra Catherine Kim Rhonda Grebe Wei Li Bryan W. Jones Donald J. Zack Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
description	Abstract The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal development, provide a platform to investigate the mechanisms of retinal degeneration and screen for neuroprotective compounds, and provide the basis for cell-based therapeutic strategies. In this study, we describe an in vitro method by which hPSCs can be differentiated into 3D retinas with at least some important features reminiscent of a mature retina, including exuberant outgrowth of outer segment-like structures and synaptic ribbons, photoreceptor neurotransmitter expression, and membrane conductances and synaptic vesicle release properties consistent with possible photoreceptor synaptic function. The advanced outer segment-like structures reported here support the notion that 3D retina cups could serve as a model for studying mature photoreceptor development and allow for more robust modeling of retinal degenerative disease in vitro.
format	article
author	Karl J. Wahlin Julien A. Maruotti Srinivasa R. Sripathi John Ball Juan M. Angueyra Catherine Kim Rhonda Grebe Wei Li Bryan W. Jones Donald J. Zack
author_facet	Karl J. Wahlin Julien A. Maruotti Srinivasa R. Sripathi John Ball Juan M. Angueyra Catherine Kim Rhonda Grebe Wei Li Bryan W. Jones Donald J. Zack
author_sort	Karl J. Wahlin
title	Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
title_short	Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
title_full	Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
title_fullStr	Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
title_full_unstemmed	Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells
title_sort	photoreceptor outer segment-like structures in long-term 3d retinas from human pluripotent stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1dd261a3fd8c4e21bc9180331f614bf5
work_keys_str_mv	AT karljwahlin photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT julienamaruotti photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT srinivasarsripathi photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT johnball photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT juanmangueyra photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT catherinekim photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT rhondagrebe photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT weili photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT bryanwjones photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells AT donaldjzack photoreceptoroutersegmentlikestructuresinlongterm3dretinasfromhumanpluripotentstemcells
_version_	1718388435504332800

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

Ejemplares similares