Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex

Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this control is assessing the activation and/or adjusting the stimulation, which requires imaging the entire volume of optogenetically-induced neuronal activity. An ideal tec...

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Autores principales:	M. Provansal, G. Labernède, C. Joffrois, A. Rizkallah, R. Goulet, M. Valet, W. Deschamps, U. Ferrari, A. Chaffiol, D. Dalkara, J. A. Sahel, M. Tanter, S. Picaud, G. Gauvain, F. Arcizet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1dd37e80ffb447ca9a7dfd8e7970b325
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spelling	oai:doaj.org-article:1dd37e80ffb447ca9a7dfd8e7970b3252021-12-02T16:04:35ZFunctional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex10.1038/s41598-021-91972-z2045-2322https://doaj.org/article/1dd37e80ffb447ca9a7dfd8e7970b3252021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91972-zhttps://doaj.org/toc/2045-2322Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this control is assessing the activation and/or adjusting the stimulation, which requires imaging the entire volume of optogenetically-induced neuronal activity. An ideal technique for this aim is fUS imaging, which allows one to generate brain-wide activation maps with submesoscopic spatial resolution. However, optical stimulation of the brain with blue light might lead to non-specific activations at high irradiances. fUS imaging of optogenetic activations can be obtained at these wavelengths using lower light power (< 2mW) but it limits the depth of directly activatable neurons from the cortical surface. Our main goal was to report that we can detect specific optogenetic activations in V1 even in deep layers following stimulation at the cortical surface. Here, we show the possibility to detect deep optogenetic activations in anesthetized rats expressing the red-shifted opsin ChrimsonR in V1 using fUS imaging. We demonstrate the optogenetic specificity of these activations and their neuronal origin with electrophysiological recordings. Finally, we show that the optogenetic response initiated in V1 spreads to downstream (LGN) and upstream (V2) visual areas.M. ProvansalG. LabernèdeC. JoffroisA. RizkallahR. GouletM. ValetW. DeschampsU. FerrariA. ChaffiolD. DalkaraJ. A. SahelM. TanterS. PicaudG. GauvainF. ArcizetNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Provansal G. Labernède C. Joffrois A. Rizkallah R. Goulet M. Valet W. Deschamps U. Ferrari A. Chaffiol D. Dalkara J. A. Sahel M. Tanter S. Picaud G. Gauvain F. Arcizet Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
description	Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this control is assessing the activation and/or adjusting the stimulation, which requires imaging the entire volume of optogenetically-induced neuronal activity. An ideal technique for this aim is fUS imaging, which allows one to generate brain-wide activation maps with submesoscopic spatial resolution. However, optical stimulation of the brain with blue light might lead to non-specific activations at high irradiances. fUS imaging of optogenetic activations can be obtained at these wavelengths using lower light power (< 2mW) but it limits the depth of directly activatable neurons from the cortical surface. Our main goal was to report that we can detect specific optogenetic activations in V1 even in deep layers following stimulation at the cortical surface. Here, we show the possibility to detect deep optogenetic activations in anesthetized rats expressing the red-shifted opsin ChrimsonR in V1 using fUS imaging. We demonstrate the optogenetic specificity of these activations and their neuronal origin with electrophysiological recordings. Finally, we show that the optogenetic response initiated in V1 spreads to downstream (LGN) and upstream (V2) visual areas.
format	article
author	M. Provansal G. Labernède C. Joffrois A. Rizkallah R. Goulet M. Valet W. Deschamps U. Ferrari A. Chaffiol D. Dalkara J. A. Sahel M. Tanter S. Picaud G. Gauvain F. Arcizet
author_facet	M. Provansal G. Labernède C. Joffrois A. Rizkallah R. Goulet M. Valet W. Deschamps U. Ferrari A. Chaffiol D. Dalkara J. A. Sahel M. Tanter S. Picaud G. Gauvain F. Arcizet
author_sort	M. Provansal
title	Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
title_short	Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
title_full	Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
title_fullStr	Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
title_full_unstemmed	Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
title_sort	functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1dd37e80ffb447ca9a7dfd8e7970b325
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Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex

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