The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid

Background: Hemoglobin (Hb), oxygen, carbon dioxide, and electron transporter of the body, may enter to an oxidation process that can convert oxyhemoglobin (oxyHb) to methemoglobin (metHb) and hemichrome. Surfactants can facilitate oxidation process that may accumulate hemichrome in red blood cells....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Radnoosh Mirzajani, Ebrahim Mirzajani, Heshmatollah Ebrahimi-Najafabadi
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2020
Materias:	hemoglobin sodium dodecyl sulfate ascorbic acid peroxide multivariate curve resolution chemometrics Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/1de293c842d54016977bd321c916bc78
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1de293c842d54016977bd321c916bc78
record_format	dspace
spelling	oai:doaj.org-article:1de293c842d54016977bd321c916bc782021-11-20T08:49:05ZThe Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid10.34172/ajmb.2020.032345-4113https://doaj.org/article/1de293c842d54016977bd321c916bc782020-06-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-8-21.pdfhttps://doaj.org/toc/2345-4113Background: Hemoglobin (Hb), oxygen, carbon dioxide, and electron transporter of the body, may enter to an oxidation process that can convert oxyhemoglobin (oxyHb) to methemoglobin (metHb) and hemichrome. Surfactants can facilitate oxidation process that may accumulate hemichrome in red blood cells. Methods: In the present study, the interaction of purified Hb with sodium dodecyl sulfate (SDS, 0-5 mM) and ascorbic acid (AA, 0-5 mM) was evaluated by UV-Vis spectroscopy and multivariate curve resolution techniques. Results: Reconstructed spectral and concentration profiles showed three forms of Hb name as oxyHb, metHb, and hemichrome with lack of fit values less than 1.85%. AA hindered the oxidation process of Hb. Conclusion: A decrease in critical micelle concentration of SDS in the presence of AA and interaction of AA with hydrogen peroxide, which is produced during the interaction of Hb with SDS, are two reasons for diminution in the oxidation process of Hb when accompanied with AA.Radnoosh MirzajaniEbrahim MirzajaniHeshmatollah Ebrahimi-NajafabadiHamadan University of Medical Sciencesarticlehemoglobinsodium dodecyl sulfateascorbic acidperoxidemultivariate curve resolutionchemometricsMedical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 8, Iss 1, Pp 21-26 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	hemoglobin sodium dodecyl sulfate ascorbic acid peroxide multivariate curve resolution chemometrics Medical technology R855-855.5
spellingShingle	hemoglobin sodium dodecyl sulfate ascorbic acid peroxide multivariate curve resolution chemometrics Medical technology R855-855.5 Radnoosh Mirzajani Ebrahim Mirzajani Heshmatollah Ebrahimi-Najafabadi The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
description	Background: Hemoglobin (Hb), oxygen, carbon dioxide, and electron transporter of the body, may enter to an oxidation process that can convert oxyhemoglobin (oxyHb) to methemoglobin (metHb) and hemichrome. Surfactants can facilitate oxidation process that may accumulate hemichrome in red blood cells. Methods: In the present study, the interaction of purified Hb with sodium dodecyl sulfate (SDS, 0-5 mM) and ascorbic acid (AA, 0-5 mM) was evaluated by UV-Vis spectroscopy and multivariate curve resolution techniques. Results: Reconstructed spectral and concentration profiles showed three forms of Hb name as oxyHb, metHb, and hemichrome with lack of fit values less than 1.85%. AA hindered the oxidation process of Hb. Conclusion: A decrease in critical micelle concentration of SDS in the presence of AA and interaction of AA with hydrogen peroxide, which is produced during the interaction of Hb with SDS, are two reasons for diminution in the oxidation process of Hb when accompanied with AA.
format	article
author	Radnoosh Mirzajani Ebrahim Mirzajani Heshmatollah Ebrahimi-Najafabadi
author_facet	Radnoosh Mirzajani Ebrahim Mirzajani Heshmatollah Ebrahimi-Najafabadi
author_sort	Radnoosh Mirzajani
title	The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
title_short	The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
title_full	The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
title_fullStr	The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
title_full_unstemmed	The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
title_sort	interaction of hemoglobin with sodium dodecyl sulfate in presence of ascorbic acid
publisher	Hamadan University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/1de293c842d54016977bd321c916bc78
work_keys_str_mv	AT radnooshmirzajani theinteractionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid AT ebrahimmirzajani theinteractionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid AT heshmatollahebrahiminajafabadi theinteractionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid AT radnooshmirzajani interactionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid AT ebrahimmirzajani interactionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid AT heshmatollahebrahiminajafabadi interactionofhemoglobinwithsodiumdodecylsulfateinpresenceofascorbicacid
_version_	1718419468888047616

The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid

Ejemplares similares