Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.

Esophageal cancer is a common tumor of the digestive system with poor prognosis. This study was to gain a better understanding of the mechanisms involved in esophageal cancer and to identify new prognostic markers. We downloaded the esophageal cancer miRNA expression profile microarray data (GSE1137...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yue Jiang, Chengda Zhang, Wenbin Shen, Yiming Li, Yun Wang, Jianjun Han, Tao Liu, Li Jia, Fei Gao, Xiaojun Liu, Mi Chen, Guangming Yi, Hongchun Dai, Jun He
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1dedeb3437bf4cedbd199e4ae3fe0310
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1dedeb3437bf4cedbd199e4ae3fe0310
record_format	dspace
spelling	oai:doaj.org-article:1dedeb3437bf4cedbd199e4ae3fe03102021-12-02T20:08:49ZIdentification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.1932-620310.1371/journal.pone.0255479https://doaj.org/article/1dedeb3437bf4cedbd199e4ae3fe03102021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255479https://doaj.org/toc/1932-6203Esophageal cancer is a common tumor of the digestive system with poor prognosis. This study was to gain a better understanding of the mechanisms involved in esophageal cancer and to identify new prognostic markers. We downloaded the esophageal cancer miRNA expression profile microarray data (GSE113740, GSE112264, GSE122497, GSE113486, and GSE106817) from the GEO database, extracted the esophageal cancer miRNA sequencing data from The Cancer Genome Atlas (TCGA) database, and then used a bioinformatics approach to select common differentially expressed miRNAs (DEMs). Differentially expressed genes (DEGs) were selected by predicting DEM target genes using the miRWalk database and intersecting with differential genes obtained from TCGA database for esophageal cancer. The STRING database was used to obtain protein-protein interaction (PPI) relationships to construct the DEM-DEG network. Furthermore, we selected core genes and core miRNAs associated with esophageal cancer prognosis by performing survival and univariate/multivariate COX analysis on DEMs and DEGs in the network and performed GSEA analysis on core genes alone, and finally the expression of the markers was verified by qPCR in esophageal cancer cell lines Eca109, SKGT-4 and normal esophageal epithelial cells HEEC. Nine DEMs were obtained, of which three were upregulated and six were downregulated, and 326 DEGs were obtained, of which 105 were upregulated and 221 were downregulated. Survival univariate/multivariate COX analysis revealed that five genes, ZBTB16, AQP4, ADCYAP1R1, PDGFD, and VIPR2, and two microRNAs, miR-99a-5p, and miR-508-5p, were related to esophageal cancer prognosis. GSEA analysis showed that the following genes may be involved in esophageal cancer prognosis: ZBTB16 may through the MTOR signaling pathway, AQP4 through the GNRH signaling pathway, ADCYAP1R1 through the PPAR signaling pathway, VIPR2 through the P53 signaling pathway and PDGFD through the PENTOSE-PHOSPHATE signaling pathway.Yue JiangChengda ZhangWenbin ShenYiming LiYun WangJianjun HanTao LiuLi JiaFei GaoXiaojun LiuMi ChenGuangming YiHongchun DaiJun HePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255479 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yue Jiang Chengda Zhang Wenbin Shen Yiming Li Yun Wang Jianjun Han Tao Liu Li Jia Fei Gao Xiaojun Liu Mi Chen Guangming Yi Hongchun Dai Jun He Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
description	Esophageal cancer is a common tumor of the digestive system with poor prognosis. This study was to gain a better understanding of the mechanisms involved in esophageal cancer and to identify new prognostic markers. We downloaded the esophageal cancer miRNA expression profile microarray data (GSE113740, GSE112264, GSE122497, GSE113486, and GSE106817) from the GEO database, extracted the esophageal cancer miRNA sequencing data from The Cancer Genome Atlas (TCGA) database, and then used a bioinformatics approach to select common differentially expressed miRNAs (DEMs). Differentially expressed genes (DEGs) were selected by predicting DEM target genes using the miRWalk database and intersecting with differential genes obtained from TCGA database for esophageal cancer. The STRING database was used to obtain protein-protein interaction (PPI) relationships to construct the DEM-DEG network. Furthermore, we selected core genes and core miRNAs associated with esophageal cancer prognosis by performing survival and univariate/multivariate COX analysis on DEMs and DEGs in the network and performed GSEA analysis on core genes alone, and finally the expression of the markers was verified by qPCR in esophageal cancer cell lines Eca109, SKGT-4 and normal esophageal epithelial cells HEEC. Nine DEMs were obtained, of which three were upregulated and six were downregulated, and 326 DEGs were obtained, of which 105 were upregulated and 221 were downregulated. Survival univariate/multivariate COX analysis revealed that five genes, ZBTB16, AQP4, ADCYAP1R1, PDGFD, and VIPR2, and two microRNAs, miR-99a-5p, and miR-508-5p, were related to esophageal cancer prognosis. GSEA analysis showed that the following genes may be involved in esophageal cancer prognosis: ZBTB16 may through the MTOR signaling pathway, AQP4 through the GNRH signaling pathway, ADCYAP1R1 through the PPAR signaling pathway, VIPR2 through the P53 signaling pathway and PDGFD through the PENTOSE-PHOSPHATE signaling pathway.
format	article
author	Yue Jiang Chengda Zhang Wenbin Shen Yiming Li Yun Wang Jianjun Han Tao Liu Li Jia Fei Gao Xiaojun Liu Mi Chen Guangming Yi Hongchun Dai Jun He
author_facet	Yue Jiang Chengda Zhang Wenbin Shen Yiming Li Yun Wang Jianjun Han Tao Liu Li Jia Fei Gao Xiaojun Liu Mi Chen Guangming Yi Hongchun Dai Jun He
author_sort	Yue Jiang
title	Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
title_short	Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
title_full	Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
title_fullStr	Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
title_full_unstemmed	Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.
title_sort	identification of serum prognostic marker mirnas and construction of microrna-mrna networks of esophageal cancer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1dedeb3437bf4cedbd199e4ae3fe0310
work_keys_str_mv	AT yuejiang identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT chengdazhang identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT wenbinshen identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT yimingli identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT yunwang identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT jianjunhan identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT taoliu identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT lijia identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT feigao identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT xiaojunliu identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT michen identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT guangmingyi identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT hongchundai identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer AT junhe identificationofserumprognosticmarkermirnasandconstructionofmicrornamrnanetworksofesophagealcancer
_version_	1718375109374246912

Identification of serum prognostic marker miRNAs and construction of microRNA-mRNA networks of esophageal cancer.

Ejemplares similares