The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.

<h4>Background</h4>Plasmid mediated antimicrobial resistance in the Enterobacteriaceae is a global problem. The rise of CTX-M class extended spectrum beta lactamases (ESBLs) has been well documented in industrialized countries. Vietnam is representative of a typical transitional middle i...

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Autores principales: Nhu Thi Khanh Nguyen, Vinh Ha, Nga Vu Thieu Tran, Richard Stabler, Duy Thanh Pham, Thi Minh Vien Le, H Rogier van Doorn, Ana Cerdeño-Tárraga, Nicholas Thomson, James Campbell, Van Minh Hoang Nguyen, Thi Thu Nga Tran, Minh Van Pham, Thuy Thu Cao, Brendan Wren, Jeremy Farrar, Stephen Baker
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Publicado: Public Library of Science (PLoS) 2010
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spelling oai:doaj.org-article:1df0278a8acb4284ab738ce753e216ed2021-12-02T20:24:26ZThe sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.1935-27271935-273510.1371/journal.pntd.0000702https://doaj.org/article/1df0278a8acb4284ab738ce753e216ed2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20544028/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Plasmid mediated antimicrobial resistance in the Enterobacteriaceae is a global problem. The rise of CTX-M class extended spectrum beta lactamases (ESBLs) has been well documented in industrialized countries. Vietnam is representative of a typical transitional middle income country where the spectrum of infectious diseases combined with the spread of drug resistance is shifting and bringing new healthcare challenges.<h4>Methodology</h4>We collected hospital admission data from the pediatric population attending the hospital for tropical diseases in Ho Chi Minh City with Shigella infections. Organisms were cultured from all enrolled patients and subjected to antimicrobial susceptibility testing. Those that were ESBL positive were subjected to further investigation. These investigations included PCR amplification for common ESBL genes, plasmid investigation, conjugation, microarray hybridization and DNA sequencing of a bla(CTX-M) encoding plasmid.<h4>Principal findings</h4>We show that two different bla(CTX-M) genes are circulating in this bacterial population in this location. Sequence of one of the ESBL plasmids shows that rather than the gene being integrated into a preexisting MDR plasmid, the bla(CTX-M) gene is located on relatively simple conjugative plasmid. The sequenced plasmid (pEG356) carried the bla(CTX-M-24) gene on an ISEcp1 element and demonstrated considerable sequence homology with other IncFI plasmids.<h4>Significance</h4>The rapid dissemination, spread of antimicrobial resistance and changing population of Shigella spp. concurrent with economic growth are pertinent to many other countries undergoing similar development. Third generation cephalosporins are commonly used empiric antibiotics in Ho Chi Minh City. We recommend that these agents should not be considered for therapy of dysentery in this setting.Nhu Thi Khanh NguyenVinh HaNga Vu Thieu TranRichard StablerDuy Thanh PhamThi Minh Vien LeH Rogier van DoornAna Cerdeño-TárragaNicholas ThomsonJames CampbellVan Minh Hoang NguyenThi Thu Nga TranMinh Van PhamThuy Thu CaoBrendan WrenJeremy FarrarStephen BakerPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 4, Iss 6, p e702 (2010)
institution DOAJ
collection DOAJ
language EN
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Nhu Thi Khanh Nguyen
Vinh Ha
Nga Vu Thieu Tran
Richard Stabler
Duy Thanh Pham
Thi Minh Vien Le
H Rogier van Doorn
Ana Cerdeño-Tárraga
Nicholas Thomson
James Campbell
Van Minh Hoang Nguyen
Thi Thu Nga Tran
Minh Van Pham
Thuy Thu Cao
Brendan Wren
Jeremy Farrar
Stephen Baker
The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
description <h4>Background</h4>Plasmid mediated antimicrobial resistance in the Enterobacteriaceae is a global problem. The rise of CTX-M class extended spectrum beta lactamases (ESBLs) has been well documented in industrialized countries. Vietnam is representative of a typical transitional middle income country where the spectrum of infectious diseases combined with the spread of drug resistance is shifting and bringing new healthcare challenges.<h4>Methodology</h4>We collected hospital admission data from the pediatric population attending the hospital for tropical diseases in Ho Chi Minh City with Shigella infections. Organisms were cultured from all enrolled patients and subjected to antimicrobial susceptibility testing. Those that were ESBL positive were subjected to further investigation. These investigations included PCR amplification for common ESBL genes, plasmid investigation, conjugation, microarray hybridization and DNA sequencing of a bla(CTX-M) encoding plasmid.<h4>Principal findings</h4>We show that two different bla(CTX-M) genes are circulating in this bacterial population in this location. Sequence of one of the ESBL plasmids shows that rather than the gene being integrated into a preexisting MDR plasmid, the bla(CTX-M) gene is located on relatively simple conjugative plasmid. The sequenced plasmid (pEG356) carried the bla(CTX-M-24) gene on an ISEcp1 element and demonstrated considerable sequence homology with other IncFI plasmids.<h4>Significance</h4>The rapid dissemination, spread of antimicrobial resistance and changing population of Shigella spp. concurrent with economic growth are pertinent to many other countries undergoing similar development. Third generation cephalosporins are commonly used empiric antibiotics in Ho Chi Minh City. We recommend that these agents should not be considered for therapy of dysentery in this setting.
format article
author Nhu Thi Khanh Nguyen
Vinh Ha
Nga Vu Thieu Tran
Richard Stabler
Duy Thanh Pham
Thi Minh Vien Le
H Rogier van Doorn
Ana Cerdeño-Tárraga
Nicholas Thomson
James Campbell
Van Minh Hoang Nguyen
Thi Thu Nga Tran
Minh Van Pham
Thuy Thu Cao
Brendan Wren
Jeremy Farrar
Stephen Baker
author_facet Nhu Thi Khanh Nguyen
Vinh Ha
Nga Vu Thieu Tran
Richard Stabler
Duy Thanh Pham
Thi Minh Vien Le
H Rogier van Doorn
Ana Cerdeño-Tárraga
Nicholas Thomson
James Campbell
Van Minh Hoang Nguyen
Thi Thu Nga Tran
Minh Van Pham
Thuy Thu Cao
Brendan Wren
Jeremy Farrar
Stephen Baker
author_sort Nhu Thi Khanh Nguyen
title The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
title_short The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
title_full The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
title_fullStr The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
title_full_unstemmed The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam.
title_sort sudden dominance of blactx-m harbouring plasmids in shigella spp. circulating in southern vietnam.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/1df0278a8acb4284ab738ce753e216ed
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