Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum

Putrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain larg...

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Autores principales:	Guangfei Tang, Haoxue Xia, Jingting Liang, Zhonghua Ma, Wende Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Fusarium graminearum polyamine biosynthesis spermidine DON (deoxynivalenol) virulence Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1df50f45c3f8469697706d7d31bbb26a
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spelling	oai:doaj.org-article:1df50f45c3f8469697706d7d31bbb26a2021-11-19T07:33:24ZSpermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum1664-302X10.3389/fmicb.2021.765398https://doaj.org/article/1df50f45c3f8469697706d7d31bbb26a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.765398/fullhttps://doaj.org/toc/1664-302XPutrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain largely obscure in plant pathogenic fungi. Fusarium graminearum causes Fusarium head blight (FHB) and leads to devastating yield losses and quality reduction by producing various kinds of mycotoxins. Herein, we genetically analyzed the gene function of the polyamine biosynthesis pathway and evaluated the role of the endogenous polyamines in the growth, development, and virulence of F. graminearum. Our results found that deletion of spermidine biosynthesis gene FgSPE3 caused serious growth defects, reduced asexual and sexual reproduction, and increased sensitivity to various stresses. More importantly, ΔFgspe3 exhibited significantly decreased mycotoxin deoxynivalenol (DON) production and weak virulence in host plants. Additionally, the growth and virulence defects of ΔFgspe3 could be rescued by exogenous application of 5 mM spermidine. Furthermore, RNA-seq displayed that FgSpe3 participated in many essential biological pathways including DNA, RNA, and ribosome synthetic process. To our knowledge, these results indicate that spermidine is essential for growth, development, DON production, and virulence in Fusarium species, which provides a potential target to control FHB.Guangfei TangGuangfei TangHaoxue XiaJingting LiangZhonghua MaWende LiuFrontiers Media S.A.articleFusarium graminearumpolyamine biosynthesisspermidineDON (deoxynivalenol)virulenceMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fusarium graminearum polyamine biosynthesis spermidine DON (deoxynivalenol) virulence Microbiology QR1-502
spellingShingle	Fusarium graminearum polyamine biosynthesis spermidine DON (deoxynivalenol) virulence Microbiology QR1-502 Guangfei Tang Guangfei Tang Haoxue Xia Jingting Liang Zhonghua Ma Wende Liu Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
description	Putrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain largely obscure in plant pathogenic fungi. Fusarium graminearum causes Fusarium head blight (FHB) and leads to devastating yield losses and quality reduction by producing various kinds of mycotoxins. Herein, we genetically analyzed the gene function of the polyamine biosynthesis pathway and evaluated the role of the endogenous polyamines in the growth, development, and virulence of F. graminearum. Our results found that deletion of spermidine biosynthesis gene FgSPE3 caused serious growth defects, reduced asexual and sexual reproduction, and increased sensitivity to various stresses. More importantly, ΔFgspe3 exhibited significantly decreased mycotoxin deoxynivalenol (DON) production and weak virulence in host plants. Additionally, the growth and virulence defects of ΔFgspe3 could be rescued by exogenous application of 5 mM spermidine. Furthermore, RNA-seq displayed that FgSpe3 participated in many essential biological pathways including DNA, RNA, and ribosome synthetic process. To our knowledge, these results indicate that spermidine is essential for growth, development, DON production, and virulence in Fusarium species, which provides a potential target to control FHB.
format	article
author	Guangfei Tang Guangfei Tang Haoxue Xia Jingting Liang Zhonghua Ma Wende Liu
author_facet	Guangfei Tang Guangfei Tang Haoxue Xia Jingting Liang Zhonghua Ma Wende Liu
author_sort	Guangfei Tang
title	Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
title_short	Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
title_full	Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
title_fullStr	Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
title_full_unstemmed	Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum
title_sort	spermidine is critical for growth, development, environmental adaptation, and virulence in fusarium graminearum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1df50f45c3f8469697706d7d31bbb26a
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Spermidine Is Critical for Growth, Development, Environmental Adaptation, and Virulence in Fusarium graminearum

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