Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge

The accumulation of toxic chemical constituents in sludge and wastewater has fuelled an interest in investigating efficient and eco-friendly wastewater remediation approaches. In this study, a set of bacterial samples were isolated from petroleum sludge and tested for their ability to degrade differ...

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Autores principales:	Manal Ali Alhefeiti, Khawlah Athamneh, Ranjit Vijayan, Syed Salman Ashraf
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	aromatic dyes bacillus cereus bioremediation emerging pollutants pseudomonas guariconensis Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/1df9ec4ab7994b3489f320769d77e94e
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spelling	oai:doaj.org-article:1df9ec4ab7994b3489f320769d77e94e2021-11-06T10:54:03ZBioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge0273-12231996-973210.2166/wst.2021.065https://doaj.org/article/1df9ec4ab7994b3489f320769d77e94e2021-04-01T00:00:00Zhttp://wst.iwaponline.com/content/83/7/1535https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The accumulation of toxic chemical constituents in sludge and wastewater has fuelled an interest in investigating efficient and eco-friendly wastewater remediation approaches. In this study, a set of bacterial samples were isolated from petroleum sludge and tested for their ability to degrade different aromatic pollutants, including azo dyes and emerging pollutants. Although exhibiting differential specificity, all bacterial isolates were able to degrade different classes of aromatic dyes efficiently. Ribosomal 16S rRNA sequencing of the 12 bacterial isolates showed that they belonged to two different bacterial genera: Bacillus cereus and Pseudomonas guariconensis. Of these 12 strains, MA1 (B. cereus) was the most promising and was chosen for further optimization and biochemical studies. The optimum culture and remediation conditions for MA1 was found to be at pH 7, with 100 ppm dye concentration, and under aerobic condition. In addition to efficiently degrading various aromatic dyes (e.g. Congo Red, Reactive Black 5, PBS, and Toluidine Blue), MA1 was also found to be capable of degrading various emerging pollutants (e.g. prometryn, fluometuron and sulfamethoxazole). Preliminary transcriptome analysis shows that MA1 grown on media containing a mixture of aromatic dyes appears to differentially express a number of genes. Data shown here strongly suggests that petroleum sludge is a rich reservoir of bacteria with powerful remediation abilities. HIGHLIGHTS Bacteria isolated from petroleum sludge can be potent agents for bioremediation.; Dye-based high-throughput approach can be used to screen for strains of interest.; B. cereus species can efficiently degrade various classes of emerging pollutants.; Whole transcriptome analyses can be used to identify genes involved in remediation.;Manal Ali AlhefeitiKhawlah AthamnehRanjit VijayanSyed Salman AshrafIWA Publishingarticlearomatic dyesbacillus cereusbioremediationemerging pollutantspseudomonas guariconensisEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 7, Pp 1535-1547 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aromatic dyes bacillus cereus bioremediation emerging pollutants pseudomonas guariconensis Environmental technology. Sanitary engineering TD1-1066
spellingShingle	aromatic dyes bacillus cereus bioremediation emerging pollutants pseudomonas guariconensis Environmental technology. Sanitary engineering TD1-1066 Manal Ali Alhefeiti Khawlah Athamneh Ranjit Vijayan Syed Salman Ashraf Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
description	The accumulation of toxic chemical constituents in sludge and wastewater has fuelled an interest in investigating efficient and eco-friendly wastewater remediation approaches. In this study, a set of bacterial samples were isolated from petroleum sludge and tested for their ability to degrade different aromatic pollutants, including azo dyes and emerging pollutants. Although exhibiting differential specificity, all bacterial isolates were able to degrade different classes of aromatic dyes efficiently. Ribosomal 16S rRNA sequencing of the 12 bacterial isolates showed that they belonged to two different bacterial genera: Bacillus cereus and Pseudomonas guariconensis. Of these 12 strains, MA1 (B. cereus) was the most promising and was chosen for further optimization and biochemical studies. The optimum culture and remediation conditions for MA1 was found to be at pH 7, with 100 ppm dye concentration, and under aerobic condition. In addition to efficiently degrading various aromatic dyes (e.g. Congo Red, Reactive Black 5, PBS, and Toluidine Blue), MA1 was also found to be capable of degrading various emerging pollutants (e.g. prometryn, fluometuron and sulfamethoxazole). Preliminary transcriptome analysis shows that MA1 grown on media containing a mixture of aromatic dyes appears to differentially express a number of genes. Data shown here strongly suggests that petroleum sludge is a rich reservoir of bacteria with powerful remediation abilities. HIGHLIGHTS Bacteria isolated from petroleum sludge can be potent agents for bioremediation.; Dye-based high-throughput approach can be used to screen for strains of interest.; B. cereus species can efficiently degrade various classes of emerging pollutants.; Whole transcriptome analyses can be used to identify genes involved in remediation.;
format	article
author	Manal Ali Alhefeiti Khawlah Athamneh Ranjit Vijayan Syed Salman Ashraf
author_facet	Manal Ali Alhefeiti Khawlah Athamneh Ranjit Vijayan Syed Salman Ashraf
author_sort	Manal Ali Alhefeiti
title	Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
title_short	Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
title_full	Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
title_fullStr	Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
title_full_unstemmed	Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge
title_sort	bioremediation of various aromatic and emerging pollutants by bacillus cereus sp. isolated from petroleum sludge
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/1df9ec4ab7994b3489f320769d77e94e
work_keys_str_mv	AT manalalialhefeiti bioremediationofvariousaromaticandemergingpollutantsbybacilluscereusspisolatedfrompetroleumsludge AT khawlahathamneh bioremediationofvariousaromaticandemergingpollutantsbybacilluscereusspisolatedfrompetroleumsludge AT ranjitvijayan bioremediationofvariousaromaticandemergingpollutantsbybacilluscereusspisolatedfrompetroleumsludge AT syedsalmanashraf bioremediationofvariousaromaticandemergingpollutantsbybacilluscereusspisolatedfrompetroleumsludge
_version_	1718443718332121088

Bioremediation of various aromatic and emerging pollutants by Bacillus cereus sp. isolated from petroleum sludge

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