X-ray inactivation of RNA viruses without loss of biological characteristics

Abstract In the event of an unpredictable viral outbreak requiring high/maximum biosafety containment facilities (i.e. BSL3 and BSL4), X-ray irradiation has the potential to relieve pressures on conventional diagnostic bottlenecks and expediate work at lower containment. Guided by Monte Carlo modell...

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Autores principales:	Babak Afrough, Jonathan Eakins, Sarah Durley-White, Stuart Dowall, Stephen Findlay-Wilson, Victoria Graham, Kuiama Lewandowski, Daniel P. Carter, Roger Hewson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1dfce6c23ba846b9863ecb3dc6b926f2
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spelling	oai:doaj.org-article:1dfce6c23ba846b9863ecb3dc6b926f22021-12-02T15:11:53ZX-ray inactivation of RNA viruses without loss of biological characteristics10.1038/s41598-020-77972-52045-2322https://doaj.org/article/1dfce6c23ba846b9863ecb3dc6b926f22020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77972-5https://doaj.org/toc/2045-2322Abstract In the event of an unpredictable viral outbreak requiring high/maximum biosafety containment facilities (i.e. BSL3 and BSL4), X-ray irradiation has the potential to relieve pressures on conventional diagnostic bottlenecks and expediate work at lower containment. Guided by Monte Carlo modelling and in vitro 1-log10 decimal-reduction value (D-value) predictions, the X-ray photon energies required for the effective inactivation of zoonotic viruses belonging to the medically important families of Flaviviridae, Nairoviridae, Phenuiviridae and Togaviridae are demonstrated. Specifically, it is shown that an optimized irradiation approach is attractive for use in a multitude of downstream detection and functional assays, as it preserves key biochemical and immunological properties. This study provides evidence that X-ray irradiation can support emergency preparedness, outbreak response and front-line diagnostics in a safe, reproducible and scalable manner pertinent to operations that are otherwise restricted to higher containment BSL3 or BSL4 laboratories.Babak AfroughJonathan EakinsSarah Durley-WhiteStuart DowallStephen Findlay-WilsonVictoria GrahamKuiama LewandowskiDaniel P. CarterRoger HewsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Babak Afrough Jonathan Eakins Sarah Durley-White Stuart Dowall Stephen Findlay-Wilson Victoria Graham Kuiama Lewandowski Daniel P. Carter Roger Hewson X-ray inactivation of RNA viruses without loss of biological characteristics
description	Abstract In the event of an unpredictable viral outbreak requiring high/maximum biosafety containment facilities (i.e. BSL3 and BSL4), X-ray irradiation has the potential to relieve pressures on conventional diagnostic bottlenecks and expediate work at lower containment. Guided by Monte Carlo modelling and in vitro 1-log10 decimal-reduction value (D-value) predictions, the X-ray photon energies required for the effective inactivation of zoonotic viruses belonging to the medically important families of Flaviviridae, Nairoviridae, Phenuiviridae and Togaviridae are demonstrated. Specifically, it is shown that an optimized irradiation approach is attractive for use in a multitude of downstream detection and functional assays, as it preserves key biochemical and immunological properties. This study provides evidence that X-ray irradiation can support emergency preparedness, outbreak response and front-line diagnostics in a safe, reproducible and scalable manner pertinent to operations that are otherwise restricted to higher containment BSL3 or BSL4 laboratories.
format	article
author	Babak Afrough Jonathan Eakins Sarah Durley-White Stuart Dowall Stephen Findlay-Wilson Victoria Graham Kuiama Lewandowski Daniel P. Carter Roger Hewson
author_facet	Babak Afrough Jonathan Eakins Sarah Durley-White Stuart Dowall Stephen Findlay-Wilson Victoria Graham Kuiama Lewandowski Daniel P. Carter Roger Hewson
author_sort	Babak Afrough
title	X-ray inactivation of RNA viruses without loss of biological characteristics
title_short	X-ray inactivation of RNA viruses without loss of biological characteristics
title_full	X-ray inactivation of RNA viruses without loss of biological characteristics
title_fullStr	X-ray inactivation of RNA viruses without loss of biological characteristics
title_full_unstemmed	X-ray inactivation of RNA viruses without loss of biological characteristics
title_sort	x-ray inactivation of rna viruses without loss of biological characteristics
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1dfce6c23ba846b9863ecb3dc6b926f2
work_keys_str_mv	AT babakafrough xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT jonathaneakins xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT sarahdurleywhite xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT stuartdowall xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT stephenfindlaywilson xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT victoriagraham xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT kuiamalewandowski xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT danielpcarter xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics AT rogerhewson xrayinactivationofrnaviruseswithoutlossofbiologicalcharacteristics
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X-ray inactivation of RNA viruses without loss of biological characteristics

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