Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and localise TARP proteins using super resolution microscop...

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Autores principales:	Diogo Bessa-Neto, Gerti Beliu, Alexander Kuhlemann, Valeria Pecoraro, Sören Doose, Natacha Retailleau, Nicolas Chevrier, David Perrais, Markus Sauer, Daniel Choquet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1dfd358afde74c72bc8f54e8fcd08c90
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spelling	oai:doaj.org-article:1dfd358afde74c72bc8f54e8fcd08c902021-11-21T12:34:57ZBioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons10.1038/s41467-021-27025-w2041-1723https://doaj.org/article/1dfd358afde74c72bc8f54e8fcd08c902021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27025-whttps://doaj.org/toc/2041-1723Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and localise TARP proteins using super resolution microscopy.Diogo Bessa-NetoGerti BeliuAlexander KuhlemannValeria PecoraroSören DooseNatacha RetailleauNicolas ChevrierDavid PerraisMarkus SauerDaniel ChoquetNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Diogo Bessa-Neto Gerti Beliu Alexander Kuhlemann Valeria Pecoraro Sören Doose Natacha Retailleau Nicolas Chevrier David Perrais Markus Sauer Daniel Choquet Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
description	Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and localise TARP proteins using super resolution microscopy.
format	article
author	Diogo Bessa-Neto Gerti Beliu Alexander Kuhlemann Valeria Pecoraro Sören Doose Natacha Retailleau Nicolas Chevrier David Perrais Markus Sauer Daniel Choquet
author_facet	Diogo Bessa-Neto Gerti Beliu Alexander Kuhlemann Valeria Pecoraro Sören Doose Natacha Retailleau Nicolas Chevrier David Perrais Markus Sauer Daniel Choquet
author_sort	Diogo Bessa-Neto
title	Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
title_short	Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
title_full	Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
title_fullStr	Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
title_full_unstemmed	Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
title_sort	bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1dfd358afde74c72bc8f54e8fcd08c90
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Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

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