Pax7 lineage contributions to the mammalian neural crest.

<h4>Background</h4>Neural crest cells are vertebrate-specific multipotent cells that contribute to a variety of tissues including the peripheral nervous system, melanocytes, and craniofacial bones and cartilage. Abnormal development of the neural crest is associated with several human ma...

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Autores principales:	Barbara Murdoch, Casey DelConte, Martín I García-Castro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e00f6d0b1b748bb86518bf8b686a5f2
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spelling	oai:doaj.org-article:1e00f6d0b1b748bb86518bf8b686a5f22021-11-18T07:10:47ZPax7 lineage contributions to the mammalian neural crest.1932-620310.1371/journal.pone.0041089https://doaj.org/article/1e00f6d0b1b748bb86518bf8b686a5f22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848431/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Neural crest cells are vertebrate-specific multipotent cells that contribute to a variety of tissues including the peripheral nervous system, melanocytes, and craniofacial bones and cartilage. Abnormal development of the neural crest is associated with several human maladies including cleft/lip palate, aggressive cancers such as melanoma and neuroblastoma, and rare syndromes, like Waardenburg syndrome, a complex disorder involving hearing loss and pigment defects. We previously identified the transcription factor Pax7 as an early marker, and required component for neural crest development in chick embryos. In mammals, Pax7 is also thought to play a role in neural crest development, yet the precise contribution of Pax7 progenitors to the neural crest lineage has not been determined.<h4>Methodology/principal findings</h4>Here we use Cre/loxP technology in double transgenic mice to fate map the Pax7 lineage in neural crest derivates. We find that Pax7 descendants contribute to multiple tissues including the cranial, cardiac and trunk neural crest, which in the cranial cartilage form a distinct regional pattern. The Pax7 lineage, like the Pax3 lineage, is additionally detected in some non-neural crest tissues, including a subset of the epithelial cells in specific organs.<h4>Conclusions/significance</h4>These results demonstrate a previously unappreciated widespread distribution of Pax7 descendants within and beyond the neural crest. They shed light regarding the regionally distinct phenotypes observed in Pax3 and Pax7 mutants, and provide a unique perspective into the potential roles of Pax7 during disease and development.Barbara MurdochCasey DelConteMartín I García-CastroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41089 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Murdoch Casey DelConte Martín I García-Castro Pax7 lineage contributions to the mammalian neural crest.
description	<h4>Background</h4>Neural crest cells are vertebrate-specific multipotent cells that contribute to a variety of tissues including the peripheral nervous system, melanocytes, and craniofacial bones and cartilage. Abnormal development of the neural crest is associated with several human maladies including cleft/lip palate, aggressive cancers such as melanoma and neuroblastoma, and rare syndromes, like Waardenburg syndrome, a complex disorder involving hearing loss and pigment defects. We previously identified the transcription factor Pax7 as an early marker, and required component for neural crest development in chick embryos. In mammals, Pax7 is also thought to play a role in neural crest development, yet the precise contribution of Pax7 progenitors to the neural crest lineage has not been determined.<h4>Methodology/principal findings</h4>Here we use Cre/loxP technology in double transgenic mice to fate map the Pax7 lineage in neural crest derivates. We find that Pax7 descendants contribute to multiple tissues including the cranial, cardiac and trunk neural crest, which in the cranial cartilage form a distinct regional pattern. The Pax7 lineage, like the Pax3 lineage, is additionally detected in some non-neural crest tissues, including a subset of the epithelial cells in specific organs.<h4>Conclusions/significance</h4>These results demonstrate a previously unappreciated widespread distribution of Pax7 descendants within and beyond the neural crest. They shed light regarding the regionally distinct phenotypes observed in Pax3 and Pax7 mutants, and provide a unique perspective into the potential roles of Pax7 during disease and development.
format	article
author	Barbara Murdoch Casey DelConte Martín I García-Castro
author_facet	Barbara Murdoch Casey DelConte Martín I García-Castro
author_sort	Barbara Murdoch
title	Pax7 lineage contributions to the mammalian neural crest.
title_short	Pax7 lineage contributions to the mammalian neural crest.
title_full	Pax7 lineage contributions to the mammalian neural crest.
title_fullStr	Pax7 lineage contributions to the mammalian neural crest.
title_full_unstemmed	Pax7 lineage contributions to the mammalian neural crest.
title_sort	pax7 lineage contributions to the mammalian neural crest.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1e00f6d0b1b748bb86518bf8b686a5f2
work_keys_str_mv	AT barbaramurdoch pax7lineagecontributionstothemammalianneuralcrest AT caseydelconte pax7lineagecontributionstothemammalianneuralcrest AT martinigarciacastro pax7lineagecontributionstothemammalianneuralcrest
_version_	1718423774493147136

Pax7 lineage contributions to the mammalian neural crest.

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