Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Neda Alvarez-Ortega, Karina Caballero-Gallardo, María Taboada-Alquerque, Jackeline Franco, Elena E. Stashenko, Cristina Juan, Ana Juan-García, Jesus Olivero-Verbel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cytotoxicity mycotoxins <i>Fridericia chica</i> protection extracts Medicine R
Acceso en línea:	https://doaj.org/article/1e06c989c891410189ab857a6e6280db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1e06c989c891410189ab857a6e6280db
record_format	dspace
spelling	oai:doaj.org-article:1e06c989c891410189ab857a6e6280db2021-11-25T19:08:32ZProtective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)10.3390/toxins131107482072-6651https://doaj.org/article/1e06c989c891410189ab857a6e6280db2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/748https://doaj.org/toc/2072-6651<i>Fridericia chica</i> (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the <i>F. chica</i> leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (<i>p</i> < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of <i>F. chica</i> grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.Neda Alvarez-OrtegaKarina Caballero-GallardoMaría Taboada-AlquerqueJackeline FrancoElena E. StashenkoCristina JuanAna Juan-GarcíaJesus Olivero-VerbelMDPI AGarticlecytotoxicitymycotoxins<i>Fridericia chica</i>protectionextractsMedicineRENToxins, Vol 13, Iss 748, p 748 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytotoxicity mycotoxins <i>Fridericia chica</i> protection extracts Medicine R
spellingShingle	cytotoxicity mycotoxins <i>Fridericia chica</i> protection extracts Medicine R Neda Alvarez-Ortega Karina Caballero-Gallardo María Taboada-Alquerque Jackeline Franco Elena E. Stashenko Cristina Juan Ana Juan-García Jesus Olivero-Verbel Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
description	<i>Fridericia chica</i> (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the <i>F. chica</i> leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (<i>p</i> < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of <i>F. chica</i> grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.
format	article
author	Neda Alvarez-Ortega Karina Caballero-Gallardo María Taboada-Alquerque Jackeline Franco Elena E. Stashenko Cristina Juan Ana Juan-García Jesus Olivero-Verbel
author_facet	Neda Alvarez-Ortega Karina Caballero-Gallardo María Taboada-Alquerque Jackeline Franco Elena E. Stashenko Cristina Juan Ana Juan-García Jesus Olivero-Verbel
author_sort	Neda Alvarez-Ortega
title	Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
title_short	Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
title_full	Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
title_fullStr	Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
title_full_unstemmed	Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)
title_sort	protective effects of the hydroethanolic extract of <i>fridericia chica</i> on undifferentiated human neuroblastoma cells exposed to α-zearalenol (α-zel) and β-zearalenol (β-zel)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1e06c989c891410189ab857a6e6280db
work_keys_str_mv	AT nedaalvarezortega protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT karinacaballerogallardo protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT mariataboadaalquerque protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT jackelinefranco protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT elenaestashenko protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT cristinajuan protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT anajuangarcia protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel AT jesusoliveroverbel protectiveeffectsofthehydroethanolicextractofifridericiachicaionundifferentiatedhumanneuroblastomacellsexposedtoazearalenolazelandbzearalenolbzel
_version_	1718410202146930688

Protective Effects of the Hydroethanolic Extract of <i>Fridericia chica</i> on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

Ejemplares similares