Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes

Introduction: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART).Data characterizing the...

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Autores principales:	Ali M Gabali, Tarek Jazaerly, Ronald Cleveland, Lawrence Kass
Formato:	article
Lenguaje:	EN
Publicado:	Thieme Medical and Scientific Publishers Pvt. Ltd. 2013
Materias:	α-naphthyl butyrate esterase cd16 cd14 cd4 human immunodeficiency virus-1 monocyte Medicine R
Acceso en línea:	https://doaj.org/article/1e09a3ccecf24a38a8fd8b19e5e66be8
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spelling	oai:doaj.org-article:1e09a3ccecf24a38a8fd8b19e5e66be82021-12-02T17:05:51ZHuman immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes2231-07702249-446410.4103/2231-0770.112787https://doaj.org/article/1e09a3ccecf24a38a8fd8b19e5e66be82013-01-01T00:00:00Zhttp://www.thieme-connect.de/DOI/DOI?10.4103/2231-0770.112787https://doaj.org/toc/2231-0770https://doaj.org/toc/2249-4464Introduction: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART).Data characterizing the morphological changes of peripheral blood monocytes in HIV-1-infected individuals are limited. Materials and Methods: In this study, we collected monocytes from 21 asymptomatic HIV-1-infected individuals with CD4 count more than 500 cells/mm 3 and healthy individuals. The monocytes ultrastructural morphologic changes and α-naphthyl butyrate esterase (ANBE) activity were compared between the two groups. Results: In monocytes from patients infected with HIV-1, activity of α?naphthyl butyrate esterase?(ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV?1?infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA?DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV?1?infected patients. -naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. Conclusions: Possibly, changes in the activity of ANBE along with a disrupted appearing cytoplasmic fibril network contribute to monocyte dysfunction in HIV-1-infected patients.Ali M GabaliTarek JazaerlyRonald ClevelandLawrence KassThieme Medical and Scientific Publishers Pvt. Ltd.articleα-naphthyl butyrate esterasecd16cd14cd4human immunodeficiency virus-1monocyteMedicineRENAvicenna Journal of Medicine, Vol 03, Iss 01, Pp 1-7 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-naphthyl butyrate esterase cd16 cd14 cd4 human immunodeficiency virus-1 monocyte Medicine R
spellingShingle	α-naphthyl butyrate esterase cd16 cd14 cd4 human immunodeficiency virus-1 monocyte Medicine R Ali M Gabali Tarek Jazaerly Ronald Cleveland Lawrence Kass Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
description	Introduction: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART).Data characterizing the morphological changes of peripheral blood monocytes in HIV-1-infected individuals are limited. Materials and Methods: In this study, we collected monocytes from 21 asymptomatic HIV-1-infected individuals with CD4 count more than 500 cells/mm 3 and healthy individuals. The monocytes ultrastructural morphologic changes and α-naphthyl butyrate esterase (ANBE) activity were compared between the two groups. Results: In monocytes from patients infected with HIV-1, activity of α?naphthyl butyrate esterase?(ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV?1?infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA?DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV?1?infected patients. -naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. Conclusions: Possibly, changes in the activity of ANBE along with a disrupted appearing cytoplasmic fibril network contribute to monocyte dysfunction in HIV-1-infected patients.
format	article
author	Ali M Gabali Tarek Jazaerly Ronald Cleveland Lawrence Kass
author_facet	Ali M Gabali Tarek Jazaerly Ronald Cleveland Lawrence Kass
author_sort	Ali M Gabali
title	Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
title_short	Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
title_full	Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
title_fullStr	Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
title_full_unstemmed	Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
title_sort	human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
publishDate	2013
url	https://doaj.org/article/1e09a3ccecf24a38a8fd8b19e5e66be8
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Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes

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