Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer

Metastasis is a complicated and only partially understood multi-step process of cancer progression. A subset of cancer cells that can leave the primary tumor, intravasate, and circulate to reach distant organs are called circulating tumor cells (CTCs). Multiple lines of evidence suggest that in meta...

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Autores principales:	Pelin Balcik-Ercin, Laure Cayrefourcq, Rama Soundararajan, Sendurai A. Mani, Catherine Alix-Panabières
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	circulating tumor cells epithelial-to-mesenchymal transition mesenchymal-to-epithelial transition metastasis-competent cells Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/1e107eab99bd4733a87a8be64fe45475
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spelling	oai:doaj.org-article:1e107eab99bd4733a87a8be64fe454752021-11-11T15:30:31ZEpithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer10.3390/cancers132154082072-6694https://doaj.org/article/1e107eab99bd4733a87a8be64fe454752021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5408https://doaj.org/toc/2072-6694Metastasis is a complicated and only partially understood multi-step process of cancer progression. A subset of cancer cells that can leave the primary tumor, intravasate, and circulate to reach distant organs are called circulating tumor cells (CTCs). Multiple lines of evidence suggest that in metastatic cancer cells, epithelial and mesenchymal markers are co-expressed to facilitate the cells’ ability to go back and forth between cellular states. This feature is called epithelial-to-mesenchymal plasticity (EMP). CTCs represent a unique source to understand the EMP features in metastatic cascade biology. Our group previously established and characterized nine serial CTC lines from a patient with metastatic colon cancer. Here, we assessed the expression of markers involved in epithelial–mesenchymal (EMT) and mesenchymal–epithelial (MET) transition in these unique CTC lines, to define their EMP profile. We found that the oncogenes MYC and ezrin were expressed by all CTC lines, but not SIX1, one of their common regulators (also an EMT inducer). Moreover, the MET activator GRHL2 and its putative targets were strongly expressed in all CTC lines, revealing their plasticity in favor of an increased MET state that promotes metastasis formation.Pelin Balcik-ErcinLaure CayrefourcqRama SoundararajanSendurai A. ManiCatherine Alix-PanabièresMDPI AGarticlecirculating tumor cellsepithelial-to-mesenchymal transitionmesenchymal-to-epithelial transitionmetastasis-competent cellsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5408, p 5408 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circulating tumor cells epithelial-to-mesenchymal transition mesenchymal-to-epithelial transition metastasis-competent cells Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circulating tumor cells epithelial-to-mesenchymal transition mesenchymal-to-epithelial transition metastasis-competent cells Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Pelin Balcik-Ercin Laure Cayrefourcq Rama Soundararajan Sendurai A. Mani Catherine Alix-Panabières Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
description	Metastasis is a complicated and only partially understood multi-step process of cancer progression. A subset of cancer cells that can leave the primary tumor, intravasate, and circulate to reach distant organs are called circulating tumor cells (CTCs). Multiple lines of evidence suggest that in metastatic cancer cells, epithelial and mesenchymal markers are co-expressed to facilitate the cells’ ability to go back and forth between cellular states. This feature is called epithelial-to-mesenchymal plasticity (EMP). CTCs represent a unique source to understand the EMP features in metastatic cascade biology. Our group previously established and characterized nine serial CTC lines from a patient with metastatic colon cancer. Here, we assessed the expression of markers involved in epithelial–mesenchymal (EMT) and mesenchymal–epithelial (MET) transition in these unique CTC lines, to define their EMP profile. We found that the oncogenes MYC and ezrin were expressed by all CTC lines, but not SIX1, one of their common regulators (also an EMT inducer). Moreover, the MET activator GRHL2 and its putative targets were strongly expressed in all CTC lines, revealing their plasticity in favor of an increased MET state that promotes metastasis formation.
format	article
author	Pelin Balcik-Ercin Laure Cayrefourcq Rama Soundararajan Sendurai A. Mani Catherine Alix-Panabières
author_facet	Pelin Balcik-Ercin Laure Cayrefourcq Rama Soundararajan Sendurai A. Mani Catherine Alix-Panabières
author_sort	Pelin Balcik-Ercin
title	Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
title_short	Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
title_full	Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
title_fullStr	Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
title_full_unstemmed	Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer
title_sort	epithelial-to-mesenchymal plasticity in circulating tumor cell lines sequentially derived from a patient with colorectal cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1e107eab99bd4733a87a8be64fe45475
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Epithelial-to-Mesenchymal Plasticity in Circulating Tumor Cell Lines Sequentially Derived from a Patient with Colorectal Cancer

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