Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.

Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from...

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Autores principales:	Nishant Singh, Alok Bhattacharya, Sudha Bhattacharya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e10a916f7ad404a9fd6dcb2df6e0d7a
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spelling	oai:doaj.org-article:1e10a916f7ad404a9fd6dcb2df6e0d7a2021-11-18T08:53:06ZHomologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.1932-620310.1371/journal.pone.0074465https://doaj.org/article/1e10a916f7ad404a9fd6dcb2df6e0d7a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098652/?tool=EBIhttps://doaj.org/toc/1932-6203Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. Here we show the up regulation of mitotic and meiotic HR genes in Entamoeba during serum starvation, and encystation. To directly demonstrate HR we use a simple PCR-based method involving inverted repeats, which gives a reliable read out, as the recombination junctions can be determined by sequencing the amplicons. Using this read out, we demonstrate enhanced HR under growth stress in E. histolytica, and during encystation in E. invadens. We also demonstrate recombination between chromosomal inverted repeats. This is the first experimental demonstration of HR in Entamoeba and will help future investigations into this process, and to explore the possibility of meiosis in Entamoeba.Nishant SinghAlok BhattacharyaSudha BhattacharyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74465 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nishant Singh Alok Bhattacharya Sudha Bhattacharya Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
description	Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. Here we show the up regulation of mitotic and meiotic HR genes in Entamoeba during serum starvation, and encystation. To directly demonstrate HR we use a simple PCR-based method involving inverted repeats, which gives a reliable read out, as the recombination junctions can be determined by sequencing the amplicons. Using this read out, we demonstrate enhanced HR under growth stress in E. histolytica, and during encystation in E. invadens. We also demonstrate recombination between chromosomal inverted repeats. This is the first experimental demonstration of HR in Entamoeba and will help future investigations into this process, and to explore the possibility of meiosis in Entamoeba.
format	article
author	Nishant Singh Alok Bhattacharya Sudha Bhattacharya
author_facet	Nishant Singh Alok Bhattacharya Sudha Bhattacharya
author_sort	Nishant Singh
title	Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
title_short	Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
title_full	Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
title_fullStr	Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
title_full_unstemmed	Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.
title_sort	homologous recombination occurs in entamoeba and is enhanced during growth stress and stage conversion.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1e10a916f7ad404a9fd6dcb2df6e0d7a
work_keys_str_mv	AT nishantsingh homologousrecombinationoccursinentamoebaandisenhancedduringgrowthstressandstageconversion AT alokbhattacharya homologousrecombinationoccursinentamoebaandisenhancedduringgrowthstressandstageconversion AT sudhabhattacharya homologousrecombinationoccursinentamoebaandisenhancedduringgrowthstressandstageconversion
_version_	1718421188622942208

Homologous recombination occurs in Entamoeba and is enhanced during growth stress and stage conversion.

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