High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.

Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice-like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction w...

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Autores principales:	Frances M Shapter, Michael Cross, Gary Ablett, Sylvia Malory, Ian H Chivers, Graham J King, Robert J Henry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e112c9d1c72449d92da704d767c62f2
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spelling	oai:doaj.org-article:1e112c9d1c72449d92da704d767c62f22021-11-18T08:41:22ZHigh-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.1932-620310.1371/journal.pone.0082641https://doaj.org/article/1e112c9d1c72449d92da704d767c62f22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24367532/?tool=EBIhttps://doaj.org/toc/1932-6203Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice-like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction with high throughput sequencing of genes for key domestication traits. While M. stipoides has previously been identified as having potential as a new grain crop for human consumption, only a limited understanding of its genetic diversity and breeding system was available to aid the domestication process. Next generation sequencing of deeply-pooled target amplicons estimated allelic diversity of a selected base population at 14.3 SNP/Mb and identified novel, putatively mutation-induced polymorphisms at about 2.4 mutations/Mb. A 97% lethal dose (LD₉₇) of ethyl methanesulfonate treatment was applied without inducing sterility in this polyploid species. Forward and reverse genetic screens identified beneficial alleles for the domestication trait, seed-shattering. Unique phenotypes observed in the M2 population suggest the potential for rapid accumulation of beneficial traits without recourse to a traditional cross-breeding strategy. This approach may be applicable to other wild species, unlocking their potential as new food, fibre and fuel crops.Frances M ShapterMichael CrossGary AblettSylvia MaloryIan H ChiversGraham J KingRobert J HenryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82641 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frances M Shapter Michael Cross Gary Ablett Sylvia Malory Ian H Chivers Graham J King Robert J Henry High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
description	Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice-like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction with high throughput sequencing of genes for key domestication traits. While M. stipoides has previously been identified as having potential as a new grain crop for human consumption, only a limited understanding of its genetic diversity and breeding system was available to aid the domestication process. Next generation sequencing of deeply-pooled target amplicons estimated allelic diversity of a selected base population at 14.3 SNP/Mb and identified novel, putatively mutation-induced polymorphisms at about 2.4 mutations/Mb. A 97% lethal dose (LD₉₇) of ethyl methanesulfonate treatment was applied without inducing sterility in this polyploid species. Forward and reverse genetic screens identified beneficial alleles for the domestication trait, seed-shattering. Unique phenotypes observed in the M2 population suggest the potential for rapid accumulation of beneficial traits without recourse to a traditional cross-breeding strategy. This approach may be applicable to other wild species, unlocking their potential as new food, fibre and fuel crops.
format	article
author	Frances M Shapter Michael Cross Gary Ablett Sylvia Malory Ian H Chivers Graham J King Robert J Henry
author_facet	Frances M Shapter Michael Cross Gary Ablett Sylvia Malory Ian H Chivers Graham J King Robert J Henry
author_sort	Frances M Shapter
title	High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title_short	High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title_full	High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title_fullStr	High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title_full_unstemmed	High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.
title_sort	high-throughput sequencing and mutagenesis to accelerate the domestication of microlaena stipoides as a new food crop.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1e112c9d1c72449d92da704d767c62f2
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High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop.

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