Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.

Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its comple...

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Autores principales:	Elena V Liyaskina, Nadezhda A Rakova, Alevtina A Kitykina, Valentina V Rusyaeva, Philip V Toukach, Alexey Fomenkov, Saulius Vainauskas, Richard J Roberts, Victor V Revin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e18ed39829e40febaa23f6a0793ea59
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spelling	oai:doaj.org-article:1e18ed39829e40febaa23f6a0793ea592021-12-02T20:09:40ZProduction and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.1932-620310.1371/journal.pone.0253482https://doaj.org/article/1e18ed39829e40febaa23f6a0793ea592021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253482https://doaj.org/toc/1932-6203Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.Elena V LiyaskinaNadezhda A RakovaAlevtina A KitykinaValentina V RusyaevaPhilip V ToukachAlexey FomenkovSaulius VainauskasRichard J RobertsVictor V RevinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253482 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena V Liyaskina Nadezhda A Rakova Alevtina A Kitykina Valentina V Rusyaeva Philip V Toukach Alexey Fomenkov Saulius Vainauskas Richard J Roberts Victor V Revin Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
description	Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.
format	article
author	Elena V Liyaskina Nadezhda A Rakova Alevtina A Kitykina Valentina V Rusyaeva Philip V Toukach Alexey Fomenkov Saulius Vainauskas Richard J Roberts Victor V Revin
author_facet	Elena V Liyaskina Nadezhda A Rakova Alevtina A Kitykina Valentina V Rusyaeva Philip V Toukach Alexey Fomenkov Saulius Vainauskas Richard J Roberts Victor V Revin
author_sort	Elena V Liyaskina
title	Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
title_short	Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
title_full	Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
title_fullStr	Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
title_full_unstemmed	Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.
title_sort	production and сharacterization of the exopolysaccharide from strain paenibacillus polymyxa 2020.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1e18ed39829e40febaa23f6a0793ea59
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Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.

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