Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology

Barnase is an extracellular ribonuclease secreted by <i>Bacillus amyloliquefaciens</i> that was originally studied as a small stable enzyme with robust folding. The identification of barnase intracellular inhibitor barstar led to the discovery of an incredibly strong protein-protein inte...

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Autores principales:	Olga Shilova, Polina Kotelnikova, Galina Proshkina, Elena Shramova, Sergey Deyev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	barnase barstar cancer therapy nanoparticles Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/1e22c4ccfb084fbea663ee1b8ad81500
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spelling	oai:doaj.org-article:1e22c4ccfb084fbea663ee1b8ad815002021-11-25T18:27:13ZBarnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology10.3390/molecules262267851420-3049https://doaj.org/article/1e22c4ccfb084fbea663ee1b8ad815002021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6785https://doaj.org/toc/1420-3049Barnase is an extracellular ribonuclease secreted by <i>Bacillus amyloliquefaciens</i> that was originally studied as a small stable enzyme with robust folding. The identification of barnase intracellular inhibitor barstar led to the discovery of an incredibly strong protein-protein interaction. Together, barnase and barstar provide a fully genetically encoded toxin-antitoxin pair having an extremely low dissociation constant. Moreover, compared to other dimerization systems, the barnase-barstar module provides the exact one-to-one ratio of the complex components and possesses high stability of each component in a complex and high solubility in aqueous solutions without self-aggregation. The unique properties of barnase and barstar allow the application of this pair for the engineering of different variants of targeted anticancer compounds and cytotoxic supramolecular complexes. Using barnase in suicide gene therapy has also found its niche in anticancer therapy. The application of barnase and barstar in contemporary experimental cancer therapy is reflected in the review.Olga ShilovaPolina KotelnikovaGalina ProshkinaElena ShramovaSergey DeyevMDPI AGarticlebarnasebarstarcancer therapynanoparticlesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6785, p 6785 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	barnase barstar cancer therapy nanoparticles Organic chemistry QD241-441
spellingShingle	barnase barstar cancer therapy nanoparticles Organic chemistry QD241-441 Olga Shilova Polina Kotelnikova Galina Proshkina Elena Shramova Sergey Deyev Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
description	Barnase is an extracellular ribonuclease secreted by <i>Bacillus amyloliquefaciens</i> that was originally studied as a small stable enzyme with robust folding. The identification of barnase intracellular inhibitor barstar led to the discovery of an incredibly strong protein-protein interaction. Together, barnase and barstar provide a fully genetically encoded toxin-antitoxin pair having an extremely low dissociation constant. Moreover, compared to other dimerization systems, the barnase-barstar module provides the exact one-to-one ratio of the complex components and possesses high stability of each component in a complex and high solubility in aqueous solutions without self-aggregation. The unique properties of barnase and barstar allow the application of this pair for the engineering of different variants of targeted anticancer compounds and cytotoxic supramolecular complexes. Using barnase in suicide gene therapy has also found its niche in anticancer therapy. The application of barnase and barstar in contemporary experimental cancer therapy is reflected in the review.
format	article
author	Olga Shilova Polina Kotelnikova Galina Proshkina Elena Shramova Sergey Deyev
author_facet	Olga Shilova Polina Kotelnikova Galina Proshkina Elena Shramova Sergey Deyev
author_sort	Olga Shilova
title	Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
title_short	Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
title_full	Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
title_fullStr	Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
title_full_unstemmed	Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology
title_sort	barnase-barstar pair: contemporary application in cancer research and nanotechnology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1e22c4ccfb084fbea663ee1b8ad81500
work_keys_str_mv	AT olgashilova barnasebarstarpaircontemporaryapplicationincancerresearchandnanotechnology AT polinakotelnikova barnasebarstarpaircontemporaryapplicationincancerresearchandnanotechnology AT galinaproshkina barnasebarstarpaircontemporaryapplicationincancerresearchandnanotechnology AT elenashramova barnasebarstarpaircontemporaryapplicationincancerresearchandnanotechnology AT sergeydeyev barnasebarstarpaircontemporaryapplicationincancerresearchandnanotechnology
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Barnase-Barstar Pair: Contemporary Application in Cancer Research and Nanotechnology

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