Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.

Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further...

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Autores principales:	Huili Zhu, Jinfeng Zhao, Rongjun Wang, Longxian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e284cbcf6504c95b4ccd30624b1eda6
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spelling	oai:doaj.org-article:1e284cbcf6504c95b4ccd30624b1eda62021-11-18T07:07:43ZMolecular identification of a rare subtype of Cryptosporidium hominis in infants in China.1932-620310.1371/journal.pone.0043682https://doaj.org/article/1e284cbcf6504c95b4ccd30624b1eda62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22937078/?tool=EBIhttps://doaj.org/toc/1932-6203Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.Huili ZhuJinfeng ZhaoRongjun WangLongxian ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43682 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huili Zhu Jinfeng Zhao Rongjun Wang Longxian Zhang Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
description	Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.
format	article
author	Huili Zhu Jinfeng Zhao Rongjun Wang Longxian Zhang
author_facet	Huili Zhu Jinfeng Zhao Rongjun Wang Longxian Zhang
author_sort	Huili Zhu
title	Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
title_short	Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
title_full	Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
title_fullStr	Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
title_full_unstemmed	Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
title_sort	molecular identification of a rare subtype of cryptosporidium hominis in infants in china.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1e284cbcf6504c95b4ccd30624b1eda6
work_keys_str_mv	AT huilizhu molecularidentificationofararesubtypeofcryptosporidiumhominisininfantsinchina AT jinfengzhao molecularidentificationofararesubtypeofcryptosporidiumhominisininfantsinchina AT rongjunwang molecularidentificationofararesubtypeofcryptosporidiumhominisininfantsinchina AT longxianzhang molecularidentificationofararesubtypeofcryptosporidiumhominisininfantsinchina
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Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.

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