Duality of β-glucan microparticles: antigen carrier and immunostimulants

Kim Baert,1 Bruno G De Geest,2 Henri De Greve,3,4 Eric Cox,1,* Bert Devriendt1,* 1Department of Virology, Parasitology and Immunology, 2Department of Pharmaceutics, Ghent University, Merelbeke, Ghent, Belgium; 3Structural Biology Research Centre, VIB, Brussels, Belgium; 4Structural Biology...

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Autores principales:	Baert K, De Geest BG, De Greve H, Cox E, Devriendt B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	β-glucan microparticles FedF antigen delivery vehicle immunostimulants Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1e299d3a841849188e3372a8851bb80a
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spelling	oai:doaj.org-article:1e299d3a841849188e3372a8851bb80a2021-12-02T01:01:58ZDuality of β-glucan microparticles: antigen carrier and immunostimulants1178-2013https://doaj.org/article/1e299d3a841849188e3372a8851bb80a2016-05-01T00:00:00Zhttps://www.dovepress.com/duality-of-beta-glucan-microparticles-antigen-carrier-and-immunostimul-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kim Baert,1 Bruno G De Geest,2 Henri De Greve,3,4 Eric Cox,1,* Bert Devriendt1,* 1Department of Virology, Parasitology and Immunology, 2Department of Pharmaceutics, Ghent University, Merelbeke, Ghent, Belgium; 3Structural Biology Research Centre, VIB, Brussels, Belgium; 4Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium *These authors contributed equally to this work Abstract: Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of β-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly 85%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific β-glucan receptor, demonstrated by blocking complement receptor 3, which is the major β-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties. Keywords: β-glucan microparticles, FedF, antigen delivery vehicle, immunostimulantsBaert KDe Geest BGDe Greve HCox EDevriendt BDove Medical Pressarticleβ-glucan microparticlesFedFantigen delivery vehicleimmunostimulantsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2463-2469 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-glucan microparticles FedF antigen delivery vehicle immunostimulants Medicine (General) R5-920
spellingShingle	β-glucan microparticles FedF antigen delivery vehicle immunostimulants Medicine (General) R5-920 Baert K De Geest BG De Greve H Cox E Devriendt B Duality of β-glucan microparticles: antigen carrier and immunostimulants
description	Kim Baert,1 Bruno G De Geest,2 Henri De Greve,3,4 Eric Cox,1,* Bert Devriendt1,* 1Department of Virology, Parasitology and Immunology, 2Department of Pharmaceutics, Ghent University, Merelbeke, Ghent, Belgium; 3Structural Biology Research Centre, VIB, Brussels, Belgium; 4Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium *These authors contributed equally to this work Abstract: Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of β-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly 85%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific β-glucan receptor, demonstrated by blocking complement receptor 3, which is the major β-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties. Keywords: β-glucan microparticles, FedF, antigen delivery vehicle, immunostimulants
format	article
author	Baert K De Geest BG De Greve H Cox E Devriendt B
author_facet	Baert K De Geest BG De Greve H Cox E Devriendt B
author_sort	Baert K
title	Duality of β-glucan microparticles: antigen carrier and immunostimulants
title_short	Duality of β-glucan microparticles: antigen carrier and immunostimulants
title_full	Duality of β-glucan microparticles: antigen carrier and immunostimulants
title_fullStr	Duality of β-glucan microparticles: antigen carrier and immunostimulants
title_full_unstemmed	Duality of β-glucan microparticles: antigen carrier and immunostimulants
title_sort	duality of β-glucan microparticles: antigen carrier and immunostimulants
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/1e299d3a841849188e3372a8851bb80a
work_keys_str_mv	AT baertk dualityofbetaglucanmicroparticlesantigencarrierandimmunostimulants AT degeestbg dualityofbetaglucanmicroparticlesantigencarrierandimmunostimulants AT degreveh dualityofbetaglucanmicroparticlesantigencarrierandimmunostimulants AT coxe dualityofbetaglucanmicroparticlesantigencarrierandimmunostimulants AT devriendtb dualityofbetaglucanmicroparticlesantigencarrierandimmunostimulants
_version_	1718403302996049920

Duality of &beta;-glucan microparticles: antigen carrier and immunostimulants

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