In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.

Type 1 diabetes is characterized by autoimmune destruction of pancreatic beta cells. The role played by autoantibodies directed against beta cells antigens in the pathogenesis of the disease is still unclear. Coxsackievirus B infection has been linked to the onset of type 1 diabetes; however its pre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Caterina Bason, Renata Lorini, Claudio Lunardi, Marzia Dolcino, Alessandro Giannattasio, Giuseppe d'Annunzio, Antonella Rigo, Nicoletta Pedemonte, Roberto Corrocher, Antonio Puccetti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e2aa64c278a464da213aea72d25eac0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1e2aa64c278a464da213aea72d25eac0
record_format	dspace
spelling	oai:doaj.org-article:1e2aa64c278a464da213aea72d25eac02021-11-18T07:55:25ZIn type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.1932-620310.1371/journal.pone.0057729https://doaj.org/article/1e2aa64c278a464da213aea72d25eac02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23469060/?tool=EBIhttps://doaj.org/toc/1932-6203Type 1 diabetes is characterized by autoimmune destruction of pancreatic beta cells. The role played by autoantibodies directed against beta cells antigens in the pathogenesis of the disease is still unclear. Coxsackievirus B infection has been linked to the onset of type 1 diabetes; however its precise role has not been elucidated yet. To clarify these issues, we screened a random peptide library with sera obtained from 58 patients with recent onset type 1 diabetes, before insulin therapy. We identified an immunodominant peptide recognized by the majority of individual patients'sera, that shares homology with Coxsackievirus B4 VP1 protein and with beta-cell specific autoantigens such as phogrin, phosphofructokinase and voltage-gated L-type calcium channels known to regulate beta cell apoptosis. Antibodies against the peptide affinity-purified from patients' sera, recognized the viral protein and autoantigens; moreover, such antibodies induced apoptosis of the beta cells upon binding the L-type calcium channels expressed on the beta cell surface, suggesting a calcium dependent mechanism. Our results provide evidence that in autoimmune diabetes a subset of anti-Coxsackievirus antibodies are able to induce apoptosis of pancreatic beta cells which is considered the most critical and final step in the development of autoimmune diabetes without which clinical manifestations do not occur.Caterina BasonRenata LoriniClaudio LunardiMarzia DolcinoAlessandro GiannattasioGiuseppe d'AnnunzioAntonella RigoNicoletta PedemonteRoberto CorrocherAntonio PuccettiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57729 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Caterina Bason Renata Lorini Claudio Lunardi Marzia Dolcino Alessandro Giannattasio Giuseppe d'Annunzio Antonella Rigo Nicoletta Pedemonte Roberto Corrocher Antonio Puccetti In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
description	Type 1 diabetes is characterized by autoimmune destruction of pancreatic beta cells. The role played by autoantibodies directed against beta cells antigens in the pathogenesis of the disease is still unclear. Coxsackievirus B infection has been linked to the onset of type 1 diabetes; however its precise role has not been elucidated yet. To clarify these issues, we screened a random peptide library with sera obtained from 58 patients with recent onset type 1 diabetes, before insulin therapy. We identified an immunodominant peptide recognized by the majority of individual patients'sera, that shares homology with Coxsackievirus B4 VP1 protein and with beta-cell specific autoantigens such as phogrin, phosphofructokinase and voltage-gated L-type calcium channels known to regulate beta cell apoptosis. Antibodies against the peptide affinity-purified from patients' sera, recognized the viral protein and autoantigens; moreover, such antibodies induced apoptosis of the beta cells upon binding the L-type calcium channels expressed on the beta cell surface, suggesting a calcium dependent mechanism. Our results provide evidence that in autoimmune diabetes a subset of anti-Coxsackievirus antibodies are able to induce apoptosis of pancreatic beta cells which is considered the most critical and final step in the development of autoimmune diabetes without which clinical manifestations do not occur.
format	article
author	Caterina Bason Renata Lorini Claudio Lunardi Marzia Dolcino Alessandro Giannattasio Giuseppe d'Annunzio Antonella Rigo Nicoletta Pedemonte Roberto Corrocher Antonio Puccetti
author_facet	Caterina Bason Renata Lorini Claudio Lunardi Marzia Dolcino Alessandro Giannattasio Giuseppe d'Annunzio Antonella Rigo Nicoletta Pedemonte Roberto Corrocher Antonio Puccetti
author_sort	Caterina Bason
title	In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
title_short	In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
title_full	In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
title_fullStr	In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
title_full_unstemmed	In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
title_sort	in type 1 diabetes a subset of anti-coxsackievirus b4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1e2aa64c278a464da213aea72d25eac0
work_keys_str_mv	AT caterinabason intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT renatalorini intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT claudiolunardi intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT marziadolcino intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT alessandrogiannattasio intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT giuseppedannunzio intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT antonellarigo intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT nicolettapedemonte intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT robertocorrocher intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells AT antoniopuccetti intype1diabetesasubsetofanticoxsackievirusb4antibodiesrecognizeautoantigensandinduceapoptosisofpancreaticbetacells
_version_	1718422716040609792

In type 1 diabetes a subset of anti-coxsackievirus B4 antibodies recognize autoantigens and induce apoptosis of pancreatic beta cells.

Ejemplares similares