Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining

Abstract In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity...

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Autores principales:	Paul J. Hurd, Kornelia Grübel, Marek Wojciechowski, Ryszard Maleszka, Wolfgang Rössler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e2b390d751a4fee83ef9f1c65b20e64
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spelling	oai:doaj.org-article:1e2b390d751a4fee83ef9f1c65b20e642021-12-02T17:04:35ZNovel structure in the nuclei of honey bee brain neurons revealed by immunostaining10.1038/s41598-021-86078-52045-2322https://doaj.org/article/1e2b390d751a4fee83ef9f1c65b20e642021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86078-5https://doaj.org/toc/2045-2322Abstract In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity indicates a novel previously unseen nuclear structure that does not colocalize with the cytoskeletal protein f-actin. A single rod-like assembly, 3.7–4.1 µm long, is present in each nucleus of KCs in adult brains of worker bees and drones with the strongest immuno-labelling found in foraging bees. In brains of young queens, the labelling is more sporadic, and the rod-like structure appears to be shorter (~ 2.1 µm). No immunostaining is detectable in worker larvae. In pupal stage 5 during a peak of brain development only some occasional staining was identified. Although the cellular function of this unexpected structure has not been determined, the unusual distinctiveness of the revealed pattern suggests an unknown and potentially important protein assembly. One possibility is that this nuclear assembly is part of the KCs plasticity underlying the brain maturation in adult honey bees. Because no labelling with this AB is detectable in brains of the fly Drosophila melanogaster and the ant Camponotus floridanus, we tentatively named this antibody AmBNSab (Apis mellifera Brain Neurons Specific antibody). Here we report our results to make them accessible to a broader community and invite further research to unravel the biological role of this curious nuclear structure in the honey bee central brain.Paul J. HurdKornelia GrübelMarek WojciechowskiRyszard MaleszkaWolfgang RösslerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paul J. Hurd Kornelia Grübel Marek Wojciechowski Ryszard Maleszka Wolfgang Rössler Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
description	Abstract In the course of a screen designed to produce antibodies (ABs) with affinity to proteins in the honey bee brain we found an interesting AB that detects a highly specific epitope predominantly in the nuclei of Kenyon cells (KCs). The observed staining pattern is unique, and its unfamiliarity indicates a novel previously unseen nuclear structure that does not colocalize with the cytoskeletal protein f-actin. A single rod-like assembly, 3.7–4.1 µm long, is present in each nucleus of KCs in adult brains of worker bees and drones with the strongest immuno-labelling found in foraging bees. In brains of young queens, the labelling is more sporadic, and the rod-like structure appears to be shorter (~ 2.1 µm). No immunostaining is detectable in worker larvae. In pupal stage 5 during a peak of brain development only some occasional staining was identified. Although the cellular function of this unexpected structure has not been determined, the unusual distinctiveness of the revealed pattern suggests an unknown and potentially important protein assembly. One possibility is that this nuclear assembly is part of the KCs plasticity underlying the brain maturation in adult honey bees. Because no labelling with this AB is detectable in brains of the fly Drosophila melanogaster and the ant Camponotus floridanus, we tentatively named this antibody AmBNSab (Apis mellifera Brain Neurons Specific antibody). Here we report our results to make them accessible to a broader community and invite further research to unravel the biological role of this curious nuclear structure in the honey bee central brain.
format	article
author	Paul J. Hurd Kornelia Grübel Marek Wojciechowski Ryszard Maleszka Wolfgang Rössler
author_facet	Paul J. Hurd Kornelia Grübel Marek Wojciechowski Ryszard Maleszka Wolfgang Rössler
author_sort	Paul J. Hurd
title	Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
title_short	Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
title_full	Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
title_fullStr	Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
title_full_unstemmed	Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
title_sort	novel structure in the nuclei of honey bee brain neurons revealed by immunostaining
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1e2b390d751a4fee83ef9f1c65b20e64
work_keys_str_mv	AT pauljhurd novelstructureinthenucleiofhoneybeebrainneuronsrevealedbyimmunostaining AT korneliagrubel novelstructureinthenucleiofhoneybeebrainneuronsrevealedbyimmunostaining AT marekwojciechowski novelstructureinthenucleiofhoneybeebrainneuronsrevealedbyimmunostaining AT ryszardmaleszka novelstructureinthenucleiofhoneybeebrainneuronsrevealedbyimmunostaining AT wolfgangrossler novelstructureinthenucleiofhoneybeebrainneuronsrevealedbyimmunostaining
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Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining

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