Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>

ABSTRACT Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the medium-chain fatty acid...

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Autores principales:	Qinxi Ma, Mihaela Ola, Elise Iracane, Geraldine Butler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Candida albicans aneuploidy fatty acids Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1e335986a3834cf6837822240ae60277
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spelling	oai:doaj.org-article:1e335986a3834cf6837822240ae602772021-11-15T15:22:20ZSusceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>10.1128/mSphere.00402-192379-5042https://doaj.org/article/1e335986a3834cf6837822240ae602772019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00402-19https://doaj.org/toc/2379-5042ABSTRACT Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the medium-chain fatty acid undecanoic acid. However, reintroducing DAL81 does not restore resistance, and editing DAL81 in a different background does not introduce sensitivity. Whole-genome sequencing revealed that the C. albicans dal81Δ/Δ strain has an extra copy of chromosomes 5 and 7. Reversion to resistance to undecanoic acid was induced by growing the sensitive strain in yeast extract-peptone-dextrose with 60 μg/ml undecanoic acid for up to 9 days. Nine isolates that regained some resistance to undecanoic acid lost one copy of chromosome 7. The copy number of chromosome 5 does not appear to affect resistance to fatty acids. Moreover, the sensitivity may be related to having two copies of haplotype B of chromosome 7. In addition, we find that C. albicans strain SN152, used to delete DAL81 and many other genes, has undergone a major loss of heterozygosity event on chromosome 2 and a smaller one on chromosome 3. IMPORTANCE Aneuploidy (changes in chromosome number) and loss of heterozygosity (LOH) occur frequently in the human-pathogenic yeast Candida albicans and are associated with adaptation to stress and to antifungal drugs. Aneuploidy and LOH can also be induced during laboratory manipulations, such as during genetic transformation. We find that C. albicans strain SN152, commonly used to generate gene deletions, has undergone a major LOH event on chromosome 2. One deletion strain generated in this background has acquired extra copies of chromosomes 5 and 7. We find that trisomy (three copies) of chromosome 7 is associated with sensitivity to fatty acids.Qinxi MaMihaela OlaElise IracaneGeraldine ButlerAmerican Society for MicrobiologyarticleCandida albicansaneuploidyfatty acidsMicrobiologyQR1-502ENmSphere, Vol 4, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans aneuploidy fatty acids Microbiology QR1-502
spellingShingle	Candida albicans aneuploidy fatty acids Microbiology QR1-502 Qinxi Ma Mihaela Ola Elise Iracane Geraldine Butler Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
description	ABSTRACT Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the medium-chain fatty acid undecanoic acid. However, reintroducing DAL81 does not restore resistance, and editing DAL81 in a different background does not introduce sensitivity. Whole-genome sequencing revealed that the C. albicans dal81Δ/Δ strain has an extra copy of chromosomes 5 and 7. Reversion to resistance to undecanoic acid was induced by growing the sensitive strain in yeast extract-peptone-dextrose with 60 μg/ml undecanoic acid for up to 9 days. Nine isolates that regained some resistance to undecanoic acid lost one copy of chromosome 7. The copy number of chromosome 5 does not appear to affect resistance to fatty acids. Moreover, the sensitivity may be related to having two copies of haplotype B of chromosome 7. In addition, we find that C. albicans strain SN152, used to delete DAL81 and many other genes, has undergone a major loss of heterozygosity event on chromosome 2 and a smaller one on chromosome 3. IMPORTANCE Aneuploidy (changes in chromosome number) and loss of heterozygosity (LOH) occur frequently in the human-pathogenic yeast Candida albicans and are associated with adaptation to stress and to antifungal drugs. Aneuploidy and LOH can also be induced during laboratory manipulations, such as during genetic transformation. We find that C. albicans strain SN152, commonly used to generate gene deletions, has undergone a major LOH event on chromosome 2. One deletion strain generated in this background has acquired extra copies of chromosomes 5 and 7. We find that trisomy (three copies) of chromosome 7 is associated with sensitivity to fatty acids.
format	article
author	Qinxi Ma Mihaela Ola Elise Iracane Geraldine Butler
author_facet	Qinxi Ma Mihaela Ola Elise Iracane Geraldine Butler
author_sort	Qinxi Ma
title	Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
title_short	Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
title_full	Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
title_fullStr	Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
title_full_unstemmed	Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>
title_sort	susceptibility to medium-chain fatty acids is associated with trisomy of chromosome 7 in <italic toggle="yes">candida albicans</italic>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/1e335986a3834cf6837822240ae60277
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Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 in <italic toggle="yes">Candida albicans</italic>

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