Structural characterisation of the Chaetomium thermophilum Chl1 helicase.

Chl1 is a member of the XPD family of 5'-3' DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1...

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Autores principales:	Zuzana Hodáková, Andrea Nans, Simone Kunzelmann, Shahid Mehmood, Ian Taylor, Frank Uhlmann, Peter Cherepanov, Martin R Singleton
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e3365f850e54ad2a6bfad153a2908e2
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spelling	oai:doaj.org-article:1e3365f850e54ad2a6bfad153a2908e22021-12-02T20:11:24ZStructural characterisation of the Chaetomium thermophilum Chl1 helicase.1932-620310.1371/journal.pone.0251261https://doaj.org/article/1e3365f850e54ad2a6bfad153a2908e22021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251261https://doaj.org/toc/1932-6203Chl1 is a member of the XPD family of 5'-3' DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1 has been shown to be required for chromosome segregation in yeast due to its role in the formation of persistent chromosome cohesion during S-phase. Here we present structural and biochemical data to show that Chl1 has the same overall domain organisation as other members of the XPD family, but with some conformational alterations. We also present data suggesting the insert domain in Chl1 regulates its DNA binding.Zuzana HodákováAndrea NansSimone KunzelmannShahid MehmoodIan TaylorFrank UhlmannPeter CherepanovMartin R SingletonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251261 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuzana Hodáková Andrea Nans Simone Kunzelmann Shahid Mehmood Ian Taylor Frank Uhlmann Peter Cherepanov Martin R Singleton Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
description	Chl1 is a member of the XPD family of 5'-3' DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1 has been shown to be required for chromosome segregation in yeast due to its role in the formation of persistent chromosome cohesion during S-phase. Here we present structural and biochemical data to show that Chl1 has the same overall domain organisation as other members of the XPD family, but with some conformational alterations. We also present data suggesting the insert domain in Chl1 regulates its DNA binding.
format	article
author	Zuzana Hodáková Andrea Nans Simone Kunzelmann Shahid Mehmood Ian Taylor Frank Uhlmann Peter Cherepanov Martin R Singleton
author_facet	Zuzana Hodáková Andrea Nans Simone Kunzelmann Shahid Mehmood Ian Taylor Frank Uhlmann Peter Cherepanov Martin R Singleton
author_sort	Zuzana Hodáková
title	Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
title_short	Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
title_full	Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
title_fullStr	Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
title_full_unstemmed	Structural characterisation of the Chaetomium thermophilum Chl1 helicase.
title_sort	structural characterisation of the chaetomium thermophilum chl1 helicase.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1e3365f850e54ad2a6bfad153a2908e2
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Structural characterisation of the Chaetomium thermophilum Chl1 helicase.

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